Platelet-Activating Factor Binding to Specific Cell Membrane Receptors

  • Frank H. Valone


Platelet-activating factor (PAF) serves as an extracellular, fluid-phase mediator of certain IgE-mediated, immune complex-mediated, and physically induced inflammatory reactions (Benveniste et al., 1972; Camuss et al., 1982; Grandel et al., 1985; Pinckard et al., 1979; Prevost et al., 1984). (For a discussion of PAF’s potential intracellular effects see Chapter 10.) Numerous studies have demonstrated that the effects of extracellular PAF are mediated by interaction of PAF with specific cell membrane receptors. Early studies demonstrated high-affinity PAF binding sites in platelets (Chesney et al., 1983; Hwang et al., 1983; Inarrea et al., 1984; Kloprogge and Akkerman., 1984; Valone et al., 1982) and neutrophils (Hwang et al., 1983; Valone and Goetzl, 1983). That these high-affinity binding sites constituted specific PAF receptors was suggested by several observations: Studies with PAF analogs demonstrated a close correlation between their potency as platelet activators and their capacity to compete with radiolabeled PAF for binding. There is a good correlation between the concentrations of PAF that elicit half-maximal cellular activation and the concentrations that half-maximally saturate PAF binding. In addition, selective platelet desensitization to PAF was associated with the loss of specific PAF binding sites (Valone et al., 1982). Nevertheless, PAF’s phospholipid structure left the existence of specific PAF receptors that mediate cellular activation somewhat in doubt. This uncertainty has largely been resolved by the development of selective PAF antagonists including CV-3988 (Terashita et al., 1983; Valone, 1985), kadsurenone (Shen et al., 1985), and BN52021 (Braquet et al., 1985).


Human Platelet Platelet Membrane Glyceryl Ether Rabbit Platelet Specific Cell Membrane Receptor 


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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Frank H. Valone
    • 1
  1. 1.Department of MedicineVeterans Administration Medical Center and the University of CaliforniaSan FransiscoUSA

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