Chemical Synthesis and Mass Spectrometry of PAF
Elucidation of the exact chemical structure of a biologically active molecule often presents a milestone in the search for understanding of the physiological or pathophysiological role such a molecule may play in biology. While the initial description of the biological activity of a substance provides the necessary interest to focus further investigations, the knowledge of the covalent structure of the substance permits, for the first time, chemical synthesis of quantities of pure material for detailed pharmacological studies, notwithstanding the proof of the identity of the biological substance. Furthermore, the knowledge of the covalent structure permits the development of analytical techniques, based on physicochemical properties, to detect and measure the molecule produced in biochemical reactions. It is this ability to measure a substance like PAF specifically, apart from biological assays, that can be the foundation of a more complete understanding of the role that this substance plays as a lipid mediator of biologic events.
KeywordsMolecular Species Chemical Synthesis Chemical Ionization Fast Atom Bombardment Ether Lipid
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