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Interrelationships in the Metabolism of Platelet-Activating Factor and Arachidonate in Neutrophils

  • Robert L. Wykle

Abstract

Polymorphonuclear neutrophils (PMN) produce PAF upon activation by a number of stimuli including phagocytosis, chemotactic peptide (fMLP), C5a, and ionophore A23187 (Camussi et al., 1981; Clark et al., 1980; Jouvin-Marche et al., 1984; Lotner et al., 1980; Lynch et al., 1979; Sánchez-Crespo et al., 1980). Of the many cells examined, PMN appear to produce the highest amount of PAF (Jouvin-Marche et al., 1984). The extent to which PMN, as well as other cells, release PAF into the surrounding milieu is controversial, although most investigators do observe release (Betz and Henson, 1980) and others have reported that approximately half the PAF synthesized is released from the cells (Jouvin-Marche et al., 1984). The release and optimal synthesis of PAF require extracellular Ca2+ (Betz and Henson, 1980, Ludwig et al., 1984, 1985). In recent studies of human PMN, it was shown that albumin added to the media enhances PAF synthesis by at least fourfold (50 mg human serum albumin/ml) and probably up to sevenfold (Ludwig et al., 1985), and that albumin is mandatory for PAF release (Ludwig et al., 1985).

Keywords

Platelet Activate Factor Human Neutrophil Glyceryl Ether Human Polymorphonuclear Leukocyte Arachidonate Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

PAF

platelet activating factor

GPC

sn-glycero-3-phosphocrioline

PMN

polymorphonuclear neutrophils

fMLP

N-formyl-methionyl-leucyl-phenylalanine

HPLC

high-performance liquid chromatography

PC

choline-containing phosphoglycerides

PE

ethanolamine-containing phosphoglycerides

HETE

5-hydroxy-6,8,ll,14-eicosatetraenoic acid

HPETE

5-hydro-peroxy-6,8,l l,14-eicosatetraenoic acid

LTB4

leukotriene B4,5(S),12(R)-dihydroxy-6-cis,8,10-trans-14-cis-eicosatetraenoic acid.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Robert L. Wykle
    • 1
  1. 1.Department of BiochemistryBowman Gray School of Medicine of Wake Forest UniversityWinston-SalemUSA

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