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Structure Analysis of Bacteriorhodopsin by Electron Crystallography

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Part of the book series: NATO ASI Series ((NSSA,volume 126))

Abstract

Bacteriorhodopsin (bR) is the protein found in the purple membrane of halobacteria, and it functions as a light-driven proton pump. It comprises a polypeptide chain of 248 amino acids and a light-absorbing chromophore retinal attached to one of the lysine residues. The protein is present in the purple membrane together with lipid molecules and forms a very well ordered two-dimensional array (Oesterhelt and Stoeckenius, 1971; Blaurock and Stoeckenius, 1971).

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References

  • Baldwin, J.M., and Henderson, R. 1984, Measurement and evaluation of electron diffraction patterns from two-dimensional crystals, Ultramicroscopy 14:319–336.

    Article  CAS  Google Scholar 

  • Blaurock, A.E., and Stoeckenius, W. 1971, Structure of purple membrane, Nature New Biol., 233:152–155.

    Article  PubMed  CAS  Google Scholar 

  • Engelman, D.M., Henderson, R., McLachlan, A.D., and Wallace, B.A. 1980, Path of the polypeptide in bacteriorhodopsin, PNAS, 77:2023–2027.

    Article  PubMed  CAS  Google Scholar 

  • Glaeser, R.M., Baldwin, J.M., Ceska T.A., and Henderson, R., Electron diffraction analysis of the M412 intermediate of bacteriorhodopsin, 1985, Biophysical J., in press.

    Google Scholar 

  • Glaeser, R.M., Jubb, J.S., and Henderson, R. 1985, Structural comparison of native and deoxycholate-treated purple membrane, Biophysical J.,in press.

    Google Scholar 

  • Hayward, S.B., and Stroud, R.M., 1981, Projected structure of purple membrane determined to 3.7Å resolution by low temperature electron microscopy, J. Mol. Biol., 151:491–517.

    Article  PubMed  CAS  Google Scholar 

  • Henderson, R., and Glaeser, R.M., 1985, Quantitative analysis of image contrast in electron micrographs of beam-sensitive crystals, Ultramicroscopy 16:139–150.

    Article  CAS  Google Scholar 

  • Henderson, R., and Shotton, D.M., 1980, Crystallisation of purple membrane in three-dimensions, J. Mol. Biol., 139:99–109.

    Article  PubMed  CAS  Google Scholar 

  • Henderson, R., and Unwin, P.N.T., 1975, Three-dimensional model of purple membrane obtained by electron microscopy, Nature 2.57:28–32.

    Article  Google Scholar 

  • Henderson, R., Baldwin, J.M., Agard, B.A., and Leifer, D., 1983, High resolution structural analysis of purple membrane, Proc. 41st EMSA, Ed. G.W. Bailey, pp. 418–421.

    Google Scholar 

  • Henderson, R., Baldwin, J.M, Downing, K.H., Lepault, J., and Zemlin, F. 1986, Structure of purple membrane from Halobacterium halobium. Recording, measurement and evaluation of electron micrographs at 3.5Å resolution, Ultramicroscopy, in press.

    Google Scholar 

  • Jubb, J.S., Worcester, D.L., Crespi, H.L., and Zaccai, G., 1984, Retinal location in purple membrane of Halobacterium halobium: a neutron diffraction study of membranes labelled in vivo with deuterated retinal, EMBO Journal, 3:1455–1461.

    PubMed  CAS  Google Scholar 

  • Katre, N.V., Finer-Moore, J., Stroud, R.M., and Hayward, S.B., 1984, Location of an extrinsic label in the primary and tertiary structure of bacteriorhodopsin, Biophysical Journal, 46:195–204.

    Article  PubMed  CAS  Google Scholar 

  • Leifer, D., and Henderson, R., 1983, Three-dimensional structure of orthorhombic purple membrane at 6Å resolution, J. Mol. Biol. 163:451–466.

    Article  PubMed  CAS  Google Scholar 

  • Michel, H., 1982, Characterisation and crystal packing of three-dimensional bacteriorhodopsin crystals, EMBO J. 1:1267–1271.

    PubMed  CAS  Google Scholar 

  • Oesterhelt, D., and Stoeckenius, W., 1971, Rhodopsin-like protein from the purple membrane of Halobacterium halobium, Nature New Biol., 233:149–152.

    PubMed  CAS  Google Scholar 

  • Seiff, F,, Wallat, I., Ermann, P., and Heyn, M.P., 1985, A neutron diffraction study on the location of the polyene chain of retinal in bacteriorhodopsin, Proc. Nat. Acad.Sci. USA, 82:3227–3231.

    Article  PubMed  CAS  Google Scholar 

  • Smith, D.J., Saxton, W.O., O’Keefe, M.A., Wood, G.J., and Stubbs, W.M., 1983, The importance of beam alignment and crystal tilt in high resolution electron microscopy, Ultramicroscopy, 11:263–282.

    Article  Google Scholar 

  • Zemlin, F., 1979, A practical procedure for alignment of a high resolution electron microscope, Ultramicroscopy, 4:241–245.

    Article  Google Scholar 

  • Zemlin, F., Weiss, K., Schiske, P., Kunath, W., and Hermann, K.H., 1978,Coma-free alignment of high resolution electron microscopes with the aid of optical diffractograms, Ultramicroscopy, 3:49–60.

    Article  Google Scholar 

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© 1987 Springer Science+Business Media New York

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Baldwin, J.M., Ceska, T.A., Glaeser, R.M., Henderson, R. (1987). Structure Analysis of Bacteriorhodopsin by Electron Crystallography. In: Moras, D., Drenth, J., Strandberg, B., Suck, D., Wilson, K. (eds) Crystallography in Molecular Biology. NATO ASI Series, vol 126. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5272-3_8

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  • DOI: https://doi.org/10.1007/978-1-4684-5272-3_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5274-7

  • Online ISBN: 978-1-4684-5272-3

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