Abstract
Bacteriorhodopsin (bR) is the protein found in the purple membrane of halobacteria, and it functions as a light-driven proton pump. It comprises a polypeptide chain of 248 amino acids and a light-absorbing chromophore retinal attached to one of the lysine residues. The protein is present in the purple membrane together with lipid molecules and forms a very well ordered two-dimensional array (Oesterhelt and Stoeckenius, 1971; Blaurock and Stoeckenius, 1971).
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References
Baldwin, J.M., and Henderson, R. 1984, Measurement and evaluation of electron diffraction patterns from two-dimensional crystals, Ultramicroscopy 14:319–336.
Blaurock, A.E., and Stoeckenius, W. 1971, Structure of purple membrane, Nature New Biol., 233:152–155.
Engelman, D.M., Henderson, R., McLachlan, A.D., and Wallace, B.A. 1980, Path of the polypeptide in bacteriorhodopsin, PNAS, 77:2023–2027.
Glaeser, R.M., Baldwin, J.M., Ceska T.A., and Henderson, R., Electron diffraction analysis of the M412 intermediate of bacteriorhodopsin, 1985, Biophysical J., in press.
Glaeser, R.M., Jubb, J.S., and Henderson, R. 1985, Structural comparison of native and deoxycholate-treated purple membrane, Biophysical J.,in press.
Hayward, S.B., and Stroud, R.M., 1981, Projected structure of purple membrane determined to 3.7Å resolution by low temperature electron microscopy, J. Mol. Biol., 151:491–517.
Henderson, R., and Glaeser, R.M., 1985, Quantitative analysis of image contrast in electron micrographs of beam-sensitive crystals, Ultramicroscopy 16:139–150.
Henderson, R., and Shotton, D.M., 1980, Crystallisation of purple membrane in three-dimensions, J. Mol. Biol., 139:99–109.
Henderson, R., and Unwin, P.N.T., 1975, Three-dimensional model of purple membrane obtained by electron microscopy, Nature 2.57:28–32.
Henderson, R., Baldwin, J.M., Agard, B.A., and Leifer, D., 1983, High resolution structural analysis of purple membrane, Proc. 41st EMSA, Ed. G.W. Bailey, pp. 418–421.
Henderson, R., Baldwin, J.M, Downing, K.H., Lepault, J., and Zemlin, F. 1986, Structure of purple membrane from Halobacterium halobium. Recording, measurement and evaluation of electron micrographs at 3.5Å resolution, Ultramicroscopy, in press.
Jubb, J.S., Worcester, D.L., Crespi, H.L., and Zaccai, G., 1984, Retinal location in purple membrane of Halobacterium halobium: a neutron diffraction study of membranes labelled in vivo with deuterated retinal, EMBO Journal, 3:1455–1461.
Katre, N.V., Finer-Moore, J., Stroud, R.M., and Hayward, S.B., 1984, Location of an extrinsic label in the primary and tertiary structure of bacteriorhodopsin, Biophysical Journal, 46:195–204.
Leifer, D., and Henderson, R., 1983, Three-dimensional structure of orthorhombic purple membrane at 6Å resolution, J. Mol. Biol. 163:451–466.
Michel, H., 1982, Characterisation and crystal packing of three-dimensional bacteriorhodopsin crystals, EMBO J. 1:1267–1271.
Oesterhelt, D., and Stoeckenius, W., 1971, Rhodopsin-like protein from the purple membrane of Halobacterium halobium, Nature New Biol., 233:149–152.
Seiff, F,, Wallat, I., Ermann, P., and Heyn, M.P., 1985, A neutron diffraction study on the location of the polyene chain of retinal in bacteriorhodopsin, Proc. Nat. Acad.Sci. USA, 82:3227–3231.
Smith, D.J., Saxton, W.O., O’Keefe, M.A., Wood, G.J., and Stubbs, W.M., 1983, The importance of beam alignment and crystal tilt in high resolution electron microscopy, Ultramicroscopy, 11:263–282.
Zemlin, F., 1979, A practical procedure for alignment of a high resolution electron microscope, Ultramicroscopy, 4:241–245.
Zemlin, F., Weiss, K., Schiske, P., Kunath, W., and Hermann, K.H., 1978,Coma-free alignment of high resolution electron microscopes with the aid of optical diffractograms, Ultramicroscopy, 3:49–60.
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© 1987 Springer Science+Business Media New York
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Baldwin, J.M., Ceska, T.A., Glaeser, R.M., Henderson, R. (1987). Structure Analysis of Bacteriorhodopsin by Electron Crystallography. In: Moras, D., Drenth, J., Strandberg, B., Suck, D., Wilson, K. (eds) Crystallography in Molecular Biology. NATO ASI Series, vol 126. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5272-3_8
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DOI: https://doi.org/10.1007/978-1-4684-5272-3_8
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