State of X-Ray Structure Determination of Ascorbate Oxidase from Green Zucchini Squash
The copper enzyme ascorbate oxidase ( E.C. 18.104.22.168 ) belongs to the group of “blue oxidases”. According to recent investigations of Avigliano et al.(1983) the enzyme has a molecular weight of 145,000 with a copper content of 8/146,000 Mr . The molecule consists of two non-covalently linked subunits of slightly different molecular weight (75,000 and 72,000 respectively) . The spectroscopic properties of ascorbate oxidase indicate that the enzyme contains all three different types of copper atoms (Fee, 1975; Malmstroem, 1982).
KeywordsAxis Parallel Ascorbate Oxidase Rotation Function Similar Packing Isomorphous Replacement
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