The Three-Dimensional Structure of Antibodies

  • Roberto J. Poljak
Part of the NATO ASI Series book series (NSSA, volume 126)


The first part of this article gives a summary of some of the most important results obtained by single-crystal X-ray diffraction techniques in the study of antibody structure. In the second part, recent result obtained in the authors’laboratory are discussed.


Antigen Binding Site Myeloma Protein Antibody Structure Bobwhite Quail California Quail 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Amzel, L.M. and Poljak, R.J., Three-dimensional structure of immunoglobulins, Ann. Rev. Biochem., 48:961 (1979).PubMedCrossRefGoogle Scholar
  2. 2.
    Davies, D.R. and Metzger, M., Structural basis of antibody function, Ann. Rev. Immunol. 1:87 (1983).CrossRefGoogle Scholar
  3. 3.
    Hill, R.L., Delaney, R., Fellow, R.E and Lebowitz, H.E., The evolutionary origins of the immunoglobulins, Proc. Natl. Acad. Sci. USA,46:1762 (1966).CrossRefGoogle Scholar
  4. 4.
    Iseuman, D.E., Dorrington, K.J. and Painter, R.H., The Structure and function of immunoglobulin domains, J. Immunol., 114:1726 (1975).Google Scholar
  5. 5.
    Kabat, E.A. and Wu, T.T., Attempts to locate complementarity- determining residues in the variable positions of light and heavy chains, Annals N.Y. Acad. Sci., 190:382 (1971).CrossRefGoogle Scholar
  6. 6.
    Poljak, R.J., Amzel, L.M., Avey, H.P., Chen, B.L., Phizackerley, R.P. and Saul, F., Three-dimensional structure of the Fab’ fragment of a human immunoglobulin at 2.8 Å resolution, Proc. Natl. Acad. Sci. USA, 70:3305 (1973).PubMedCrossRefGoogle Scholar
  7. 7.
    Segal, D.M., Padlan, E.A., Cohen, G.H., Rudikoff, S., Potter, M. and Davies, D.R., The three-dimensional structure of a phosphorylcholine binding mouse immunoglobulin Fab and the nature of the antigen binding site, Proc. Natl. Acad. Sc i. USA, 71:4298 (1974).CrossRefGoogle Scholar
  8. 8.
    Schiffer, M., Girling, R.L., Ely, K.R. and Edmundson, A.B. Structure of a type Bence-Jones protein at 3.5 A resolution, Biochemistry, 12:4620 (1973).PubMedCrossRefGoogle Scholar
  9. 9.
    Epp, O., Colman, P., Fehlhammer, H., Bode, W., Schiffer, M. and Huber, R., Crystal and molecular structure of a dimer composed of the variable portions of the Bence-Jones Protein Rei, Eur. J. Biochem., 45:513 (1974).PubMedCrossRefGoogle Scholar
  10. 10.
    Tonegawa, S., Somatic generation of antibody diversity, Nature, 302:575 (1983).PubMedCrossRefGoogle Scholar
  11. 11.
    Honjo, T., Immunoglobulin genes, Ann. Rev. Immunol., 1:499 (1983).CrossRefGoogle Scholar
  12. 12.
    Hood, L., Campbell, J.H. and Elgin, S.C.R., The organization, expression and evolution of antibody genes and other multigene families, Ann. Rev. Genetics, 9:305 (1975).CrossRefGoogle Scholar
  13. 13.
    Amzel, L.M., Poljak, R.J., Saul, F., Varga, J.M., and Richards, F.F., The three-dimensional structure of a combining region-ligand complex of immunoglobulin New at 35 Å resolution, Proc. Natl. Acad. Sci. USA, 71:1427 (1974).PubMedCrossRefGoogle Scholar
  14. 14.
    Köhler, G. and Milstein, C., Continuous cultures of fused cells secreting antibody of predefined specificity, Nature, 256: 495 (1975).PubMedCrossRefGoogle Scholar
  15. 15.
    Amit., A.G., Mariuzza, R.A., Saludjian, P., Poljak, R.J., Lamoyi, E. and Nisonoff, A., Preliminary crystallographic study of the Fab fragment of a monoclonal anti-phenylarsonate antibody possessing a major idiotypic specificity, J. Mol. Biol., 169:637 (1983).PubMedCrossRefGoogle Scholar
  16. 16.
    Amit, A.G., Harper, M., Mariuzza, R.A., Saludjian, P., Poljak, R.J., Lamoyi, E., and Nisonoff, A., Preliminary crystallographic study of the Fab fragment of a monoclonal anti-phenylarsonate antibody, J. Mol. Biol., 165:415 (1983).PubMedCrossRefGoogle Scholar
  17. 17.
    Mariuzza, R.A., Boulot, G., Guillon, V., Poljak, R.J., Berek, C., Jarvis, J.M. and Milstein, C, Preliminary crystallographic study of the Fab fragments of two monoclonal anti-2-phenyl-oxazolone antibodies, J Biol. Chem. (in press, 1985).Google Scholar
  18. 18.
    Mariuzza, R.A., Jankovic, D.Lj., Boulot, G., Amit, A.G., Saludjian, P., Le Guern, A., Mazié, J.C. and Poljak, R.J., Preliminary crystallographic study of the complex between the Fab fragment of a monoclonal anti-lysozyme antibody and its antigen, J. Mol. Biol., 170:1055 (1983).PubMedCrossRefGoogle Scholar
  19. 19.
    Amit, A.G., Mariuzza, R.A., Phillips, S.E.V. and Poljak, R.J., The three-dimensional structure of an antigen-antibody complex at 6 1 resolution, Nature, 133:156 (1985).CrossRefGoogle Scholar
  20. 20.
    Benjamin, D.C., Berzofsky, J.A., East, I.J., Gurd, F.R.N., Hannum, C, Leach, S.J., Margoliash, E., Michael, J.G., Miller, A., Prager, E.M., Reichlin, M., Sercarz, E.E., Smith-Gill, S.J., Todd, P.E. and Wilson, A.C., The antigenic structure of proteins: a reappraisal, Ann. Rev. Immunol., 2:67 ( 1984).CrossRefGoogle Scholar
  21. 21.
    Blake, C.C.F., Koening, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 A resolution, Nature, 206:757 (1965).PubMedCrossRefGoogle Scholar
  22. 22.
    Atassi, M.Z., Precise determination of the entire antigenic structure of lysozyme: molecular features of protein antigenic structures and potential of “surface-simulation synthesis” -a powerful new concept for protein binding sites, Immunochem. 15:909 (1978).CrossRefGoogle Scholar
  23. 23.
    Ibrahimi, I.M., Eder, J., Prager, E.M., Wilson, A.C. and Arnon, R., The effect of a single amino acid substitution on the antigenic specificity of the loop region of lysozyme, Mol. Immunol., 17:37 (1980).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Roberto J. Poljak
    • 1
  1. 1.Département d’ImmunologieInstitut PasteurParis Cedex 15France

Personalised recommendations