Abstract
The first part of this article gives a summary of some of the most important results obtained by single-crystal X-ray diffraction techniques in the study of antibody structure. In the second part, recent result obtained in the authors’laboratory are discussed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Amzel, L.M. and Poljak, R.J., Three-dimensional structure of immunoglobulins, Ann. Rev. Biochem., 48:961 (1979).
Davies, D.R. and Metzger, M., Structural basis of antibody function, Ann. Rev. Immunol. 1:87 (1983).
Hill, R.L., Delaney, R., Fellow, R.E and Lebowitz, H.E., The evolutionary origins of the immunoglobulins, Proc. Natl. Acad. Sci. USA,46:1762 (1966).
Iseuman, D.E., Dorrington, K.J. and Painter, R.H., The Structure and function of immunoglobulin domains, J. Immunol., 114:1726 (1975).
Kabat, E.A. and Wu, T.T., Attempts to locate complementarity- determining residues in the variable positions of light and heavy chains, Annals N.Y. Acad. Sci., 190:382 (1971).
Poljak, R.J., Amzel, L.M., Avey, H.P., Chen, B.L., Phizackerley, R.P. and Saul, F., Three-dimensional structure of the Fab’ fragment of a human immunoglobulin at 2.8 Å resolution, Proc. Natl. Acad. Sci. USA, 70:3305 (1973).
Segal, D.M., Padlan, E.A., Cohen, G.H., Rudikoff, S., Potter, M. and Davies, D.R., The three-dimensional structure of a phosphorylcholine binding mouse immunoglobulin Fab and the nature of the antigen binding site, Proc. Natl. Acad. Sc i. USA, 71:4298 (1974).
Schiffer, M., Girling, R.L., Ely, K.R. and Edmundson, A.B. Structure of a type Bence-Jones protein at 3.5 A resolution, Biochemistry, 12:4620 (1973).
Epp, O., Colman, P., Fehlhammer, H., Bode, W., Schiffer, M. and Huber, R., Crystal and molecular structure of a dimer composed of the variable portions of the Bence-Jones Protein Rei, Eur. J. Biochem., 45:513 (1974).
Tonegawa, S., Somatic generation of antibody diversity, Nature, 302:575 (1983).
Honjo, T., Immunoglobulin genes, Ann. Rev. Immunol., 1:499 (1983).
Hood, L., Campbell, J.H. and Elgin, S.C.R., The organization, expression and evolution of antibody genes and other multigene families, Ann. Rev. Genetics, 9:305 (1975).
Amzel, L.M., Poljak, R.J., Saul, F., Varga, J.M., and Richards, F.F., The three-dimensional structure of a combining region-ligand complex of immunoglobulin New at 35 Å resolution, Proc. Natl. Acad. Sci. USA, 71:1427 (1974).
Köhler, G. and Milstein, C., Continuous cultures of fused cells secreting antibody of predefined specificity, Nature, 256: 495 (1975).
Amit., A.G., Mariuzza, R.A., Saludjian, P., Poljak, R.J., Lamoyi, E. and Nisonoff, A., Preliminary crystallographic study of the Fab fragment of a monoclonal anti-phenylarsonate antibody possessing a major idiotypic specificity, J. Mol. Biol., 169:637 (1983).
Amit, A.G., Harper, M., Mariuzza, R.A., Saludjian, P., Poljak, R.J., Lamoyi, E., and Nisonoff, A., Preliminary crystallographic study of the Fab fragment of a monoclonal anti-phenylarsonate antibody, J. Mol. Biol., 165:415 (1983).
Mariuzza, R.A., Boulot, G., Guillon, V., Poljak, R.J., Berek, C., Jarvis, J.M. and Milstein, C, Preliminary crystallographic study of the Fab fragments of two monoclonal anti-2-phenyl-oxazolone antibodies, J Biol. Chem. (in press, 1985).
Mariuzza, R.A., Jankovic, D.Lj., Boulot, G., Amit, A.G., Saludjian, P., Le Guern, A., Mazié, J.C. and Poljak, R.J., Preliminary crystallographic study of the complex between the Fab fragment of a monoclonal anti-lysozyme antibody and its antigen, J. Mol. Biol., 170:1055 (1983).
Amit, A.G., Mariuzza, R.A., Phillips, S.E.V. and Poljak, R.J., The three-dimensional structure of an antigen-antibody complex at 6 1 resolution, Nature, 133:156 (1985).
Benjamin, D.C., Berzofsky, J.A., East, I.J., Gurd, F.R.N., Hannum, C, Leach, S.J., Margoliash, E., Michael, J.G., Miller, A., Prager, E.M., Reichlin, M., Sercarz, E.E., Smith-Gill, S.J., Todd, P.E. and Wilson, A.C., The antigenic structure of proteins: a reappraisal, Ann. Rev. Immunol., 2:67 ( 1984).
Blake, C.C.F., Koening, D.F., Mair, G.A., North, A.C.T., Phillips, D.C. and Sarma, V.R., Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 A resolution, Nature, 206:757 (1965).
Atassi, M.Z., Precise determination of the entire antigenic structure of lysozyme: molecular features of protein antigenic structures and potential of “surface-simulation synthesis” -a powerful new concept for protein binding sites, Immunochem. 15:909 (1978).
Ibrahimi, I.M., Eder, J., Prager, E.M., Wilson, A.C. and Arnon, R., The effect of a single amino acid substitution on the antigenic specificity of the loop region of lysozyme, Mol. Immunol., 17:37 (1980).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Springer Science+Business Media New York
About this chapter
Cite this chapter
Poljak, R.J. (1987). The Three-Dimensional Structure of Antibodies. In: Moras, D., Drenth, J., Strandberg, B., Suck, D., Wilson, K. (eds) Crystallography in Molecular Biology. NATO ASI Series, vol 126. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5272-3_33
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5272-3_33
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5274-7
Online ISBN: 978-1-4684-5272-3
eBook Packages: Springer Book Archive