The Structure of Poliovirus at 2.9 Å Resolution: Crystallographic Methods and Biological Implications
Poliovirus is a member of the Picornavirus family, which also includes a number of biologically significant pathogens such as hepatitis A virus, foot and mouth disease virus, the coxsackie viruses, and the rhinoviruses. The poliovirus capsid is approximately 300 A in diameter, with a mass of 8.4 million daltons. It is composed of sixty copies each of the four capsid proteins VP1 , VP2, VP3, and VP4 (approximately 33, 30, 26, and 7.5 kilodaltons, respectively), arranged to form a T=l icosahedral shell. The capsid encloses one molecule of single stranded message sense RNA of approximately 7500 nucleotides (2.5 million daltons)1. In nature, there are three known stable serotypes of poliovirus, and within each serotype there is a large number of strains. We have solved the structure of the Mahoney strain of type 1 poliovirus at 2.9 Å resolution2. Recently, Rossmann et al.3have reported the structure of a closely related virus, rhinovirus 14.
KeywordsCapsid Protein Plant Virus Antigenic Site Carboxy Terminus Major Capsid Protein
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