The Role of Separate Domains and of Individual Amino-Acids in Enzyme Catalysis, Studied by Site-Directed Mutagenesis
Protein engineering is the production of a novel or altered protein — a ‘factitious’ protein — by the creation or manipulation of a gene which controls its synthesis, for a specific purpose. To achieve the intended purpose, an ability to predict the properties of the factitious protein is required.
KeywordsTP51 Mutant Protein Engineering Factitious Protein Protein Crystallography Main Chain Conformation
Unable to display preview. Download preview PDF.
- Bhat, T.N. &Blow, D.M. 1982, Acta Cryst. A38 : 21–29.Google Scholar
- Bhat, T.N. &Blow, D.M. 1983, Acta Cryst. A39 : 166–170.Google Scholar
- Blow, D.M. &Brick, P. 1985, Aminoacyl-tRNA Synthetases, in: “Biological Macromolecules and Assemblies. Vol. II”,, F.A. Jurnak &A. McPherson, ed., Wiley, New York.Google Scholar
- Konnert, J.H. &Hendrickson, W.A. (1980), Acta Cryst. A36 : 344–350.Google Scholar
- Waye, M.M.Y., Winter, G., Wilkinson, A.J. &Fersht, A.R. 1983, EMB0 J. 2 :1827–1829.Google Scholar