Enzyme Mechanism: What X-Ray Crystallography can(not) Tell Us

  • Johan N. Jansonius
Part of the NATO ASI Series book series (NSSA, volume 126)


Any claim that X-ray crystallography can make a contribution towards the elucidation of enzyme mechanisms implies that the spatial structure of a protein in a crystal lattice corresponds to that in solution. Is that the case? This question, which regularly has been asked (and is still being asked), has been considered by e.g. Drenth (1972), Matthews (1977), and Fersht (1977). All gave an affirmative answer.


Carbonyl Carbon Enzyme Mechanism Oxyanion Hole Tetrahedral Intermediate Thiol Protease 
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Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Johan N. Jansonius
    • 1
  1. 1.Department of Structural BiologyBiozentrum University of BaselBaselSwitzerland

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