Enzyme Mechanism: What X-Ray Crystallography can(not) Tell Us
Any claim that X-ray crystallography can make a contribution towards the elucidation of enzyme mechanisms implies that the spatial structure of a protein in a crystal lattice corresponds to that in solution. Is that the case? This question, which regularly has been asked (and is still being asked), has been considered by e.g. Drenth (1972), Matthews (1977), and Fersht (1977). All gave an affirmative answer.
KeywordsCarbonyl Carbon Enzyme Mechanism Oxyanion Hole Tetrahedral Intermediate Thiol Protease
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