Investigation of the Sensitivity of Oriented (φX-174 DNA to Ionizing Radiation
in specific reduced dichroism changes which saturate at a field strength of 15,000 V/cm.
in increased sensitivity to damage by gamma-radiation estimated by agarose gel electrophoresis as single strand breaks.
Due to the mobility of the DNA, the maximum voltage used was 400 (2400 V/cm) and the percentage increase in yield (of SSBs per molecule) was 38%. At this field strength, theory predicts that only about 10% of the molecules are oriented by the field. The increase in damage must therefore be largely attributed to the conformational changes (unfolding) in the molecules induced by the applied electric field.
KeywordsField Strength External Electric Field Single Strand Break Radiation Experiment Electric Field Effect
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