A Method for the Detection of Neo-Antigens in X-ray Induced Thymomas of C57BL/6 Mouse
Retroviruses are often encountered in radio-induced (X-rays) thymomas of the C57BL/6 mouse. Several viral strains, collectively referred to as “RadLVs ”, were shown, upon injection to normal mice to mimic the “radio-induced disease ”. However, the hypothesis of the role of radiations in activating endogenous non-pathogenic retroviruses leading (via recombination) to pathogenic RadLVs which in turn would be the etiological agents of the disease is not demonstrated. Indeed, RadLVs were detected in only a few instances.
Thus, instead of searching systematic association between tumor induction and virus expression, we tried to reveal radio-induced tumor antigens whatever their origin, viral or cellular. For this, we looked if radio-induced tumors harbored antigen(s) that could be recognized by gammaglobulins produced in the irradiated animal itself. Because of the need of large amounts of such specific immunoglobulins, we hybridized the own lymphocytes of the diseased animal with myeloma cells in order to obtain monoclonal antibodies (MoAb). As determined by ELISA, a large number of hybridoma cultures were found to secrete specific tumor antibodies.
When a number of such clonal antibodies were assayed for their ability to recognized different tumor cell extracts, it was found that only a few of them were specific of only one extract whereas the other cross-reacted with variable numbers of tumors. This was taken as an indication that radio-induced antigens were polymorphic in nature and differently expressed in distinct tumors.
KeywordsSucrose Lymphoma Leukemia Recombination Titration
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