Chemical Inhibition of the Repair of DNA Single Strand Breaks Produced by X-irradiation or Hydrogen Peroxide in Cultured Mammalian Cells

  • O. Cantoni
  • F. Cattabeni
Part of the NATO ASI Series book series (NSSA, volume 124)


The effect of various metal compounds, formaldehyde and adriamycin on the rejoining of X-rays induced DNA single strand breaks was examined using the alkaline elution technique. of the compounds tested, Chromate, mercury (II) and formaldehyde decreased the ability of cells to rejoin DNA strand breaks. The inhibition of DNA repair could be demonstrated also using another system where strand breaks were produced by exposing cells for 1 hr. to 30 HM H2O2, at ice temperature.

NiCl2, CoCl2, Pb(C2H3O2)2 Cr(C2H3O2)3, CdCl2 and adriamycin were not effective in inhibiting strand break rejoining.

The possible biological significance of DNA repair inhibition is discussed.


Strand Break Chinese Hamster Ovary Cell Metal Compound Unirradiated Cell Repair Inhibition 
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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • O. Cantoni
    • 1
  • F. Cattabeni
    • 1
  1. 1.Istituto di Farmacologia e FarmacognosiaUniversita degli Studi di UrbinoUrbinoItaly

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