Chemical Inhibition of the Repair of DNA Single Strand Breaks Produced by X-irradiation or Hydrogen Peroxide in Cultured Mammalian Cells
The effect of various metal compounds, formaldehyde and adriamycin on the rejoining of X-rays induced DNA single strand breaks was examined using the alkaline elution technique. of the compounds tested, Chromate, mercury (II) and formaldehyde decreased the ability of cells to rejoin DNA strand breaks. The inhibition of DNA repair could be demonstrated also using another system where strand breaks were produced by exposing cells for 1 hr. to 30 HM H2O2, at ice temperature.
NiCl2, CoCl2, Pb(C2H3O2)2 Cr(C2H3O2)3, CdCl2 and adriamycin were not effective in inhibiting strand break rejoining.
The possible biological significance of DNA repair inhibition is discussed.
KeywordsStrand Break Chinese Hamster Ovary Cell Metal Compound Unirradiated Cell Repair Inhibition
Unable to display preview. Download preview PDF.
- 4.K. W. Kohn, R. A. G. Ewig, L. C. Erickson, and L. A. Zwelling, in: “DNA Repair — A Laboratory Manual of Research Procedures,” E. Friedberg and P. C. Hanawalt, eds., Vol. 1, Part B (1981) pp. 378.Google Scholar
- 5.O. Cantoni and M. Costa, Molecular Pharmacol. 24:84 (1983).Google Scholar
- 6.O. Cantoni, D. Murray, and R. Meyn, Biochem. Biophys. Acta. In press.Google Scholar
- 8.O. Cantoni, P. Sestiei and F. Cattabeni, Bull. Environ. Cont. Tox. In Press.Google Scholar
- 10.U. Ringborg and B. Lambert, in: “DNA Repair Late Effects,” H. Altmann, H. Riklis, and H. Slor (1980), pp. 311.Google Scholar
- 12.M. Tanaka and S. Yoshida, Biochem. 87:911 (1980).Google Scholar