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Alterations in Benzo(a)Pyrene Metabolism and its DNA Adduct Formation in Skin of Mice Chronically Exposed to Ultraviolet-B Radiation

  • H. Mukhtar
  • M. Das
  • D. R. Bickers
Part of the NATO ASI Series book series (NSSA, volume 124)

Abstract

Cutaneous xenobiotic metabolizing enzymes including aryl hydrocarbon hydroxylase (AHH), 7-ethoxycoumarin 0-deethylase (ECD), epoxide hydrolase (EH) and glutathione S-transferase (GST) activities were examined in SKH hairless mice chronically irradiated with UVB to induce squamous cell carcinoma (SCC). Enzyme activities in irradiated tumor-bearing skin were compared to those present in the skin of non-irradiated control animals as well as in unirradiated non-tumor bearing skin sites of the SCC-bearing mice. The inducibility of skin AHH and ECD in each set of animals was assessed following a single topical application of coal tar (1 ml/100 gm). Enzyme-mediated binding of 3H-benzo(a)pyrene (BP) and its metabolite 7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE-I) to epidermal DNA was also evaluated. Basal AHH and ECD activities in microsomes for UVB-irradiated SCC-bearing dorsal skin were 4.6 and 4.8-fold lower than those in dorsal skin of non-irradiated control animals. Enzyme activities in non-tumor bearing ventral skin from the UVB-irradiated SCC-bearing mice also were 2.2-2.8-fold lower as compared to activities in. the non-irradiated control animals. The reduction in AHH activity paralleled the levels of enzyme-mediated binding of radiolabeled BP metabolites and of BPDE-I to epidermal DNA. GST activity was found to be increased (173% in non-tumor bearing ventral skin of UVB-irradiated mice whereas no difference in activity between SCC-bearing dorsal skin and dorsal skin of sese control animals could be detected. EH activity was unchanged in each group of animals. Treatment with topically applied coal tar resulted in higher inducibility of AHH and ECU in both SCC-bearing (13-fold) as well as in non-tumor skin sites (6-fold) of UVB-irradiated mice than in skin of control animals (3-fold). Coal tar application also increased the covalent binding of 3H-BP and of the metabolite BPDE-I to skin DNA. This was greater in SCC-bearing dorsal skin (119–129%) than in non-irradiated skin of control animals (48–162%). Our studies suggest that the metabolism of BP by cutaneous cytochrome P-450 dependent monooxygenases is impaired in skin of mice irradiated chronically with UVB. These studies also illustrate the complex interrelationship that exist in target tissue simultaneously exposed to chemical and physical oncogens in the environment.

Keywords

Covalent Binding Epoxide Hydrolase Dorsal Skin Hairless Mouse Aryl Hydrocarbon Hydroxylase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AHH

aryl hydrocarbon hydroxylase

EH

epoxide hydrolase

ECD

7-ethoxycoumarin O-deethylase

GST

glutathione S-transferase

BP

benzo(a)pyrene

3-OH BP

3-hydroxybenzo(a)pyrene

BP-4

5-oxide, benzo(a)pyrene 4,5-oxide

BPDE-I

73,8α-dihydroxy-9α, 10 α-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene

UVB

ultraviolet B, CDNB, 1-chloro 2,4-dinitrobenzene

NADH

nicotinamide adenine dinucleotide reduced

NADPH

nicotinamide adenine dinucleotide phosphate reduced

SCC

squamous cell carcinoma

PAH

polycyclic aromatic hydrocarbon

PBS

phosphate buffered saline

ELISA

enzyme linked immunoabsorbent assay

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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • H. Mukhtar
    • 1
    • 2
  • M. Das
    • 1
    • 2
  • D. R. Bickers
    • 1
    • 2
  1. 1.Department of DermatologyUniversity Hospitals of Cleveland Case Western Reserve UniversityClevelandUSA
  2. 2.Veterans Administration Medical CenterClevelandUSA

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