Radiation Induced Transformation in Primary Differentiated Thyroid Cultures
A technique has been developed where long-term differentiated cell cultures can be established from human and animal (sheep) thyroid glands. The cultures retain morphological and functional characteristics of in vivo thyroid tissue. These include iodide trapping, T4 production and PAS positive follicle development. The cultures have been irradiated and subcultured to provide data on survival following exposure to various doses of125 Iodide or60 Cobalt. More recently, a technique has been developed which allows the development of several endpoints of in vitro transformation to be monitored in the irradiated cultures.
The system provides one of the first opportunities to study radiation transformation in primary differentiated cultures of epithelial origin.
KeywordsAgar Lactate Cobalt Iodine Hydrocortisone
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- 1.R. E. Albert, F. J. Burns, Carcinogenic atmospheric pollutants and the nature of low level risk, in: “Origins of Human Cancer, Vol. 4, Book A,” H. H. Hiatt, J. D. Watson, and J. A. Winsten, eds., Cold Spring Harbor Lab., Cold Spring Harbor, New York (1977), pp. 289–292.Google Scholar
- 3.J. E. Dumont, J. F. Malone, and A. J. Van Herle, “Irradiation and Thyroid Disease: Dosimetric, Clinical and Carcinogenic Aspects,” Commission of the European Communities Publication No. Eur 6713 en. (1980).Google Scholar
- 5.E. J. Hall, H. H. Rossi, M. Zaider, R. C. Miller, and C. Borek, in: “Neutron Carcinogenesis,” J. J. Broerse and G. B. Gerber, eds., Commission of the European Communities, Luxemburg (1982), pg. 381–405.Google Scholar
- 7.J. C. Klein, The use of in vitro methods for the study of X-ray induced transformation, in: “Biology of Radiation Carcinogenesis,” J. M. Yuhas, R. W. Tennant, and J. D. Regan, eds., Raven Press, New York (1976), p. 301.Google Scholar
- 8.P. F. Kruse and M. K. Patterson, “Tissue Culture: Methods and Applications,” Academic Press, New York (1973).Google Scholar
- 9.R. Land, L. F. Parada, and R. A. Weinberg, Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes, Nature 304:597–602.Google Scholar
- 10.R. G. Martin and J. L. Anderson, Death and transformation, in: “Biology of Radiation Carcinogenesis,” J. M. Yuhas, R. W. Tennant, and J. D. Regan, eds, Raven Press, New York (1976), p. 287Google Scholar
- 16.A. Murphy, C. Mothersill, M. K. O’Connor, J. F. Malone, M. J, Cullen, and J. K. Taaffe, An investigation of the optimum culture conditions for a differentiated culture of sheep thyroid cells, Acta Endocrinoligica 104:431–436 (1983).Google Scholar
- 19.M. K. O’ Connor, M. J. Cullen, and J. F. Malone, Long-term culture of sheep thyroid cells, Acta Endocrinol. 93, Suppl. 231 (1980).Google Scholar
- 21.J. Paul, “Cell and Tissue Culture, 5 Edition,” Churchill Livingstone, Edinburgh, New York and London (1965).Google Scholar