Radiation Induced Transformation in Primary Differentiated Thyroid Cultures

  • C. B. Seymour
  • C. Mothersill
Part of the NATO ASI Series book series (NSSA, volume 124)


A technique has been developed where long-term differentiated cell cultures can be established from human and animal (sheep) thyroid glands. The cultures retain morphological and functional characteristics of in vivo thyroid tissue. These include iodide trapping, T4 production and PAS positive follicle development. The cultures have been irradiated and subcultured to provide data on survival following exposure to various doses of125 Iodide or60 Cobalt. More recently, a technique has been developed which allows the development of several endpoints of in vitro transformation to be monitored in the irradiated cultures.

The system provides one of the first opportunities to study radiation transformation in primary differentiated cultures of epithelial origin.


Thyroid Cell Cloning Efficiency Lactate Dehydro Human Thyroid Cell Irradiate Culture 
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  1. 1.
    R. E. Albert, F. J. Burns, Carcinogenic atmospheric pollutants and the nature of low level risk, in: “Origins of Human Cancer, Vol. 4, Book A,” H. H. Hiatt, J. D. Watson, and J. A. Winsten, eds., Cold Spring Harbor Lab., Cold Spring Harbor, New York (1977), pp. 289–292.Google Scholar
  2. 2.
    B. J. Duffy, Jr. and P. J. Fitzgerald, Cancer of the thyroid in children, a report of 28 cases. Journal of Clinical Endocrinology 10: 1296 (1955).CrossRefGoogle Scholar
  3. 3.
    J. E. Dumont, J. F. Malone, and A. J. Van Herle, “Irradiation and Thyroid Disease: Dosimetric, Clinical and Carcinogenic Aspects,” Commission of the European Communities Publication No. Eur 6713 en. (1980).Google Scholar
  4. 4.
    T. Ezaki, K. Yaguwa, Y. Hayashi, T. Nishida and T. Ishmaru, J. Jap. Pract. Surg. Soc. 144(9): 1127 (1983).CrossRefGoogle Scholar
  5. 5.
    E. J. Hall, H. H. Rossi, M. Zaider, R. C. Miller, and C. Borek, in: “Neutron Carcinogenesis,” J. J. Broerse and G. B. Gerber, eds., Commission of the European Communities, Luxemburg (1982), pg. 381–405.Google Scholar
  6. 6.
    A. R. Kennedy, J. Cairns, and J. B. Little, Timing of the steps in transformation of C3H 10T1/2 cells by X-irradiation, Nature 307:85–86 (1984).PubMedCrossRefGoogle Scholar
  7. 7.
    J. C. Klein, The use of in vitro methods for the study of X-ray induced transformation, in: “Biology of Radiation Carcinogenesis,” J. M. Yuhas, R. W. Tennant, and J. D. Regan, eds., Raven Press, New York (1976), p. 301.Google Scholar
  8. 8.
    P. F. Kruse and M. K. Patterson, “Tissue Culture: Methods and Applications,” Academic Press, New York (1973).Google Scholar
  9. 9.
    R. Land, L. F. Parada, and R. A. Weinberg, Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes, Nature 304:597–602.Google Scholar
  10. 10.
    R. G. Martin and J. L. Anderson, Death and transformation, in: “Biology of Radiation Carcinogenesis,” J. M. Yuhas, R. W. Tennant, and J. D. Regan, eds, Raven Press, New York (1976), p. 287Google Scholar
  11. 11.
    C. Mothersill, A. Murphy, M. K. O’Connor, C. B. Seymour, and J. F. Malone, A role for lactate in the differentiation of cultured sheep thyroid cells, Cell Biol. Int. Reports 5(9):877–886 (1981).CrossRefGoogle Scholar
  12. 12.
    C. Mothersill, C. B. Seymour, and J. F. Malone, Maintenance of differentiated sheep thyroid cells in culture for 3 months, Acta Endocrinologica 107:54–59 (1984).PubMedGoogle Scholar
  13. 13.
    C. Mothersill, C. B. Seymour, M.J. Moriarty and M.J. Cullen, Longterm culture of human differentiated thyroid cells, Acta Endocrinologica, 108:192–199 (1985).PubMedGoogle Scholar
  14. 14.
    C. Mothersill and C. Seymour, Development of transformed characteristics by sheep thyroid cells irradiated as differentiated primary cultures, Cell Biol. Int. Reports, 8(10):887–896 (1984).CrossRefGoogle Scholar
  15. 15.
    R. T. Mulcahy, M. N. Gould, and K. H. Clifton, Radiogenic initiation of thyroid cancer: a common cellular event, Int. J. Radiat. Biol. 45(5):419–426 (1984).CrossRefGoogle Scholar
  16. 16.
    A. Murphy, C. Mothersill, M. K. O’Connor, J. F. Malone, M. J, Cullen, and J. K. Taaffe, An investigation of the optimum culture conditions for a differentiated culture of sheep thyroid cells, Acta Endocrinoligica 104:431–436 (1983).Google Scholar
  17. 17.
    R. F. Newbold and R. W. Overell, Fibroblast immortality is a prerequisite for transformation by EJ c-Ha-ras oncogenes, Nature 304:648–651 (1983).PubMedCrossRefGoogle Scholar
  18. 18.
    R. F. Newbold, R. W. Overell, and J. F. Connell, Induction of immortality is an early event in malignant transformation of mammalian cells by carcinogens, Nature 229:633–635 (1982).CrossRefGoogle Scholar
  19. 19.
    M. K. O’ Connor, M. J. Cullen, and J. F. Malone, Long-term culture of sheep thyroid cells, Acta Endocrinol. 93, Suppl. 231 (1980).Google Scholar
  20. 20.
    L. N. Parker, J. L. Belsky, T. Mandai, W. Blot and R. Kawate, Serum thyrotropin level and goitre in relation to childhood exposure to atomic radiation. J. Clin. Endocrinol. & Metab. 37: 797 (1973).CrossRefGoogle Scholar
  21. 21.
    J. Paul, “Cell and Tissue Culture, 5 Edition,” Churchill Livingstone, Edinburgh, New York and London (1965).Google Scholar
  22. 22.
    T. T. Puck and P. I. Marcus, Action of X-rays on mammalian cells, J. Exp. Med. 103:653–666 (1956).PubMedCrossRefGoogle Scholar
  23. 23.
    C. A. Reznikoff, J. S. Bertram, D. W. Brankow, and C. Heidelberger, Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to post-confluence inhibition of division, Cancer Res. 33:3239 (1973).PubMedGoogle Scholar
  24. 23.
    E. R. Rooney and R. W. Powell, Carcinoma of the thyroid in children after Xray therapy in early childhood. J. Am. Med. Assoc. 169: 1 (1959).PubMedCrossRefGoogle Scholar
  25. 24.
    C. L. Simpson and L. H. Hemplemann, The association of tumours and Roentgen ray treatment of the thorax in infancy. Cancer 10: 42 (1957).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • C. B. Seymour
    • 1
  • C. Mothersill
    • 1
  1. 1.Saint Luke’s Hospital RathgarDublin 6Ireland

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