Radiation Induced Transformation in Primary Differentiated Thyroid Cultures

  • C. B. Seymour
  • C. Mothersill
Part of the NATO ASI Series book series (NSSA, volume 124)

Abstract

A technique has been developed where long-term differentiated cell cultures can be established from human and animal (sheep) thyroid glands. The cultures retain morphological and functional characteristics of in vivo thyroid tissue. These include iodide trapping, T4 production and PAS positive follicle development. The cultures have been irradiated and subcultured to provide data on survival following exposure to various doses of125 Iodide or60 Cobalt. More recently, a technique has been developed which allows the development of several endpoints of in vitro transformation to be monitored in the irradiated cultures.

The system provides one of the first opportunities to study radiation transformation in primary differentiated cultures of epithelial origin.

Keywords

Agar Lactate Cobalt Iodine Hydrocortisone 

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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • C. B. Seymour
    • 1
  • C. Mothersill
    • 1
  1. 1.Saint Luke’s Hospital RathgarDublin 6Ireland

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