The Effect of AH 5183 (2-(4-Phenylpiperidino)-Cyclohexanol) on Acetylcholine Synthesis and Release in the Isolated Guinea Pig Ileum Longitudinal Muscle/Myenteric Plexus Preparation
AH 5183 (2-(4-phenylpiperidino)-cyclohexanol) has been shown by Anderson et al. (1983) to be a potent inhibitor of the active transport of ACh into synaptic vesicles isolated from the electric organ of Torpedo. Toll and Howard (1980) and Melega and Howard (1984) reported that AH 5183 inhibited the loading of ACh into storage vesicles in PC 12 cells without affecting the vesicular storage of preformed ACh. They found no effect on choline uptake or ACh synthesis. Studies on synaptosomes and slices from rat and mouse brain (Otero et al., 1985; Carroll, 1985; Jope and Johnson, 1986) and perfused superior cervical ganglion of the cat (Collier et al., in press) have also shown an inhibition of evoked ACh release, with associated changes in Ch transport, ACh synthesis and levels. A recent electrophysiological study (Vyskocil, 1985) concluded that non-quantal leakage of ACh in mouse diaphragm was also inhibited by AH 5183.
KeywordsMyenteric Plexus Electric Organ Choline Uptake Control Preparation Acetylcholine Synthesis
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