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The Effect of AH 5183 (2-(4-Phenylpiperidino)-Cyclohexanol) on Acetylcholine Synthesis and Release in the Isolated Guinea Pig Ileum Longitudinal Muscle/Myenteric Plexus Preparation

  • Donald J. Jenden
  • Margareth Roch
  • Ruth A. Booth
  • Kathleen M. Rice
  • Georgette M. Buga

Abstract

AH 5183 (2-(4-phenylpiperidino)-cyclohexanol) has been shown by Anderson et al. (1983) to be a potent inhibitor of the active transport of ACh into synaptic vesicles isolated from the electric organ of Torpedo. Toll and Howard (1980) and Melega and Howard (1984) reported that AH 5183 inhibited the loading of ACh into storage vesicles in PC 12 cells without affecting the vesicular storage of preformed ACh. They found no effect on choline uptake or ACh synthesis. Studies on synaptosomes and slices from rat and mouse brain (Otero et al., 1985; Carroll, 1985; Jope and Johnson, 1986) and perfused superior cervical ganglion of the cat (Collier et al., in press) have also shown an inhibition of evoked ACh release, with associated changes in Ch transport, ACh synthesis and levels. A recent electrophysiological study (Vyskocil, 1985) concluded that non-quantal leakage of ACh in mouse diaphragm was also inhibited by AH 5183.

Keywords

Myenteric Plexus Electric Organ Choline Uptake Control Preparation Acetylcholine Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Donald J. Jenden
    • 1
  • Margareth Roch
    • 1
  • Ruth A. Booth
    • 1
  • Kathleen M. Rice
    • 1
  • Georgette M. Buga
    • 1
  1. 1.Department of Pharmacology and Brain Research Institute School of MedicineCenter for the Health Sciences University of CaliforniaLos AngelesUSA

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