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The Application of Immunochemical Techniques to the Study of Cholinergic Function

  • Victor P Whittaker
  • Edilio Borroni

Abstract

The electromotor system of the electric ray, Torpedo marmovata, is shown diagrammatically in Fig. la. The electrocytes of the electric organ develop embryologically from myotubes (Fig. lb)1,2. Their ventral (under) surfaces are almost completely (80%) covered with synapses resembling - not unexpectedly - neuromuscular junctions; the transmitter is acetylcholine3. A single average-sized fish contains about 400 g of electric organ which in turn contains 500–1000 times more synaptic material per unit weight than muscle; thus electric organ is an ideal starting material for biochemical investigations of cholinergic function.

Keywords

Synaptic Vesicle Cholinergic Neurone Synaptosomal Plasma Membrane Choline Acetyl Presynaptic Plasma Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    G.Q. Fox and G.P. Richardson, J. Comp. Neurol. 179: 677 (1978)PubMedCrossRefGoogle Scholar
  2. 2.
    G.Q. Fox and G.P. Richardson, J. Comp. Neurol. 185- 293 (1979)Google Scholar
  3. 3.
    W. Feldberg and A. Fessard, J. Physiol. 101: 200 (1942)PubMedGoogle Scholar
  4. 4.
    D. Schraid, Abstr. Colloquium on Biochemie des Nervensystems, Ges. f. biol. Chemie, Göttingen, Dec 1984Google Scholar
  5. 5.
    G.P. Richardson, B. Rinschen and G.Q. Fox, J. Comp. Neurol.. 231: 339 (1985)Google Scholar
  6. 6.
    L.P. Davies, V.P. Whittaker and H. Zimmermann, Exp. Brain Res. 30: 493 (1977)PubMedCrossRefGoogle Scholar
  7. 7.
    H. Stadler and M.-L. Kiene, Abstr. Colloquium on ‘Biochemie des Nervensystems’, Ges. f. biol. Chemie, Göttingen, Dec 1984Google Scholar
  8. 8.
    W.D. Krenz, T. Tashiro, K. Wächtler, V.P. Whittaker and V. Witzemann, Neuroscience 5: 617 (1980)PubMedCrossRefGoogle Scholar
  9. 9.
    M.N. Sheridan, V.P. Whittaker and M. Israël, Z. Zellforsch. 74: 291 (1966)CrossRefGoogle Scholar
  10. 10.
    V.P. Whittaker, W.B. Essman and G.H.C. Dowe, Bioehem. J.. 128: 833 (1972)Google Scholar
  11. 11.
    T. Tashiro and H. Stadler, Eur. J. Bioehem. 90: 479 (1978)CrossRefGoogle Scholar
  12. 12.
    K. Ohsawa, G.H.C. Dowe, S.J. Morris and V.P. Whittaker, Brain Res. 161: 447 (1979)PubMedCrossRefGoogle Scholar
  13. 13.
    M.J. Dowdall, G.Q. Fox, K. Wächtler, V.P. Whittaker and H. Zimmermann, Cold Spring Harbor Symp. quant. Biol. 40: 65 (1975)CrossRefGoogle Scholar
  14. 14.
    M.J. Dowdall and H. Zimmermann; Neuroscience 2: 405 (1977)PubMedCrossRefGoogle Scholar
  15. 15.
    M. Israël, R. Manaranche, P. Mastour-Franchon and N. Morel, Bio ehern. J.. 160: 113 (1976)Google Scholar
  16. 16.
    H. Stadler and T. Tashiro, Eur. J. Bioehem. 101: 171 (1979)CrossRefGoogle Scholar
  17. 17.
    I. Ducis and V.P. Whittaker, Biochim. Biophys. Acta (in press)Google Scholar
  18. 18.
    J.H. Walker, R.T. Jones, J. Obrocki, G.P. Richardson and H. Stadler, Cell Tiss. Res. 223: 101 (1982)CrossRefGoogle Scholar
  19. 19.
    J.H. Walker, J. Obrocki and C.W. Zimmermann, J. Neuroehem. 41: 209 (1983)CrossRefGoogle Scholar
  20. 20.
    N.N. Osborne, R. Beale, D. Nicholas, H. Stadler, J.H. Walker, R.T. Jones and V.P. Whittaker, Cell. Mol. Neurobiol. 2: 157 (1982)CrossRefGoogle Scholar
  21. 21.
    R.T. Jones, J.H. Walker, P.J. Richardson, G.Q. Fox and V.P. Whittaker, Cell Tiss. Res.. 218: 355 (1981)CrossRefGoogle Scholar
  22. 22.
    P.J. Richardson, J.H. Walker, R.T. Jones and V.P. Whittaker, J. Neuroehem. 38: 1605 (1982)CrossRefGoogle Scholar
  23. 23.
    P. Ferretti and E. Borroni, submitted for publicationGoogle Scholar
  24. 24.
    V.P. Whittaker, E. Borroni, P. Ferretti and G.P. Richardson, in: Antibody Combining Sites: their Investigation and Exploitation in Subcellular Studies, E. Heid, ed., Plenum Press, New York (1985)Google Scholar
  25. 25.
    E. Borroni and J. Obrocki, unpublished resultsGoogle Scholar
  26. 26.
    D.A. Lee and V. Witzemann, Biochemistry 22: 6123 (1983)PubMedCrossRefGoogle Scholar
  27. 27.
    H. Stadler and E.M. Fenwick, Eur. J. Biochem.. 136: 377 (1983)PubMedCrossRefGoogle Scholar
  28. 28.
    K. Zechel and H. Stadler, J. Neurochem. 39: 788 (1982)PubMedCrossRefGoogle Scholar
  29. 29.
    H. Stadler and V.P. Whittaker, Brain Res. 153: 408 (1978)PubMedCrossRefGoogle Scholar
  30. 30.
    H. Stadler and G.H.C. Dowe, EMB0 J. 1: 1381 (1982)Google Scholar
  31. 31.
    H. Stadler, Colloquium on ‘Neurochemie’, Ges. f. biol. Chemie, Mosbach, April 1985Google Scholar
  32. 32.
    H. Zimmermann and V.P. Whittaker, Nature, Lond., 267: 633 (1977)CrossRefGoogle Scholar
  33. 33.
    H. Zimmermann and C.R. Denston, Neuroscience 2: 715 (1977)PubMedCrossRefGoogle Scholar
  34. 34.
    H. Zimmermann and C.R. Denston, Neuroscience 2: 695 (1977)CrossRefGoogle Scholar
  35. 35.
    P. E. Giompres, H. Zimmermann and V.P. Whittaker, Neuroscience 6: 775 (1981)PubMedCrossRefGoogle Scholar
  36. 36.
    V. P. Whittaker, G.H.C. Dowe and I.S. Roed, unpublished resultsGoogle Scholar
  37. 37.
    P. E. Giompres and V.P. Whittaker, Biochim. Biophys. Acta 770: 166 (1984)CrossRefGoogle Scholar
  38. 38.
    V.P. Whittaker, Biochera. Soc. Trans. 12: 561 (1984)Google Scholar
  39. 39.
    V. P. Whittaker, G.H.C. Dowe, J.H. Walker and I.S. Roed, unpublished resultsGoogle Scholar
  40. 40.
    R. T. Jones, J.H. Walker, H. Stadler and V.P. Whittaker, Cell Tiss. Res. 223: 117 (1982)Google Scholar
  41. 41.
    H. Zimmermann and V.P. Whittaker, J. Neurochem. 12: 435 (1974)CrossRefGoogle Scholar
  42. 42.
    R. R. Baker and M.J. Dowdall, Neurochem. Res. 1: 153 (1976)CrossRefGoogle Scholar
  43. 43.
    M. Weiler, I.S. Roed and V.P. Whittaker, J. Neurochem. 38: 1187 (1982)PubMedCrossRefGoogle Scholar
  44. 44.
    N. Morel, M. Israel, R. Manaranche and P. Mastour-Franchon, J. Cell Biol. 75: 43 (1977)PubMedCrossRefGoogle Scholar
  45. 45.
    N. Morel, M. Israel, R. Manaranche and B. Lesbats, Prog. Brain Res. 49: 191 (1979)PubMedCrossRefGoogle Scholar
  46. 46.
    H. Zimmermann, M.J. Dowdall and D.A. Lane, Neuroscience 4: 979 (1979)PubMedCrossRefGoogle Scholar
  47. 47.
    P. J. Richardson and V.P. Whittaker, J. Neurochem. 36: 1536 (1981)PubMedCrossRefGoogle Scholar
  48. 48.
    F. M. Meunier, J. Physiol. 354: 121 (1984)PubMedGoogle Scholar
  49. 49.
    P. J. Richardson, J. Neurochem. 37: 35 8 (1981)Google Scholar
  50. 50.
    P. J. Richardson, J. Neurochem. 41: 640 (1983)PubMedCrossRefGoogle Scholar
  51. 51.
    V. P. Whittaker, in: Handbook of Neurochemistry, A. Lajtha, ed., vol 7, pp 1 - 69, Plenum Press, New York (1984)Google Scholar
  52. 52.
    E. Borroni, unpublished resultsGoogle Scholar
  53. 53.
    P. J. Richardson, H. Siddle and J.P. Luzio, Biochem. J. 219: 643 (1984)Google Scholar
  54. 54.
    V. P. Whittaker, Prog. Brain Res.. 45: 45 (1976)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Victor P Whittaker
    • 1
  • Edilio Borroni
    • 1
  1. 1.Abteilung NeurochemieMax-Planck-Institut für biophysikalische ChemieGöttingenGermany

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