Development of the Acetylcholine Receptor Clusters Induced by Basic Polypeptides in Cultured Muscle Cells

  • H. Benjamin Peng


Vertebrate neuromuscular junction is marked by a concentration of acetylcholine receptors (AChR) in the postsynaptic membrane. From studies using electron–microscopic (EM) autoradiography, it has been estimated that there are over ten thousand AChR per square micron within the AChR cluster (1). This concentration of AChR is essential for the efficiency of the synaptic transmission and is the key event in the development of the postjunctional membrane. Tissue culture of neurons and muscle cells has provided a simple and convenient system to study this process. In monolayer cultures, the growth of the neurons and the establishment of the synaptic connection can be followed with high–resolution light microscopy and electron microscopy. The ease of being able to manipulate the preparation also gives it tremendous advantage in physiological and pharmacological studies over the in vivo systems.


Acetylcholine Receptor Neuromuscular Junction Cluster Process Postsynaptic Membrane Latex Bead 


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  1. 1.
    Fertuck, H.C., and M.M. Salpeter. 1976. Quantitation of junctional and extrajunctional acglcholine receptors by electron microscope autoradiography after l–of–bungarotoxin binding at mouse neuromuscular junctions. J Cell Biol. 69: 144–158.PubMedCrossRefGoogle Scholar
  2. 2.
    Anderson, M.J., and M.W. Cohen. 1977. Nerve–induced and spontaneous redistribution of acetylcholine receptors on cultured muscle cells. PhysioK (LondJ 268: 757–773.Google Scholar
  3. 3.
    Frank, E., and G.D. Fischbach. 1979. Early events in neuromuscular junction formation in vitro. JL Cell Biol. 83: 143–158.CrossRefGoogle Scholar
  4. 4.
    Podleski, T.R., D. Axelrod, P. Ravdin, I. Greenberg, M.M. Johnson, and M.M. Salpeter. 1978. Nerve extract induces increase and redistribution of acetylcholine receptors on cultured muscle colls. Proc. NatL, Acad. ScjL 75: 2035–2039.Google Scholar
  5. 5.
    Jessell, T.M., R.E. Siege, and G.D. Fischbach. 1979. Induction of acetylcholine receptors on cultured skeletal muscle by a factor extracted from brain and spinal cord. Proc. Natl. AcacL Sci. USA. 76: 5397–5401.Google Scholar
  6. 6.
    Christian, C.N., M.P. Daniels, H. Sugiyama, Z. Vogel, L. Jacques, and P.G. Nelson. 1978. A factor from neurons increases the number of acetylcholine receptor aggregates on cultured muscle cells. Proc. Najtl Acad. Sci… USA. 75: 4011–4015.Google Scholar
  7. 7.
    Schaffner, A.E., and M.P. Daniels. 1982. Conditioned medium from cultures of embryonic neurons contains a high molecular weight factor which induces acetylcholine receptor aggregation on cultured myotubes. J Neurosci 2: 623–632.PubMedGoogle Scholar
  8. 8.
    Peng, H. B., P.–C. Cheng, and P. W. Luther. 1981. Formation of ACh receptor clusters induced by positively charged latex beads. Nature 292: 831–834.PubMedCrossRefGoogle Scholar
  9. 9.
    Peng, H. B., and P.–C. Cheng. 1982. Formation of postsynaptic specializations induced by latex beads in cultured muscle cells. J Neurpsci 2: 1760–1774.Google Scholar
  10. 10.
    Anderson, M.J., M.W. Cohen, and E. Zorychta. 1977. Effects of innervation on the distribution of acetylcholine receptors on cultured muscle cells. J Physiol. lLond.J 268: 731–756.Google Scholar
  11. 11.
    Peng, H.B., and Y. Nakajima. 1978. Membrane particle aggregates in innervated and noninnervated cultures of Xenogus embryonic muscle cells. Proc Natl. AcacL Sci. USA. 75: 500–5047Google Scholar
  12. 12.
    Ravdin, P., and D. Axelrod. 1977. Fluorescent tetramethyl rhodamine derivatives of a–bungarotoxin: preparation, separation and charaterization. Anal. Biochem. 80: 585–592.Google Scholar
  13. 13.
    Peng, H.B. 1984. Participation pf calcium and calmodulin in the formation of acetylcholine receptor clusters. J Cell Biij 98:550– 557.Google Scholar
  14. 14.
    Peng, H.B., and K.A. Phelan. 1984. Early cytoplasmic specialization at the presumptive acetylcholine receptor cluster: a meshwork of thin filaments. Jv Cell Biol. 99: 344–349.CrossRefGoogle Scholar
  15. 15.
    Burry, R.W., D.A. Kniss, and L.R. Seribner. 1984. Mechanisms of synapse formation and maturation. In Current topics in research on synapses, Ed. E.G.Jones. Vol. 1, pp. 1–51. Allan R. Liss, Inc., New York.Google Scholar
  16. 16.
    Froehner, S.C. 1984. Peripheral proteins of postsynaptic membranes from Torpedo electric organ identified with monoclonal antibodies. ¿JL Cell BioI 99: 88–96.Google Scholar
  17. 17.
    Froehner, S.C., V. Gulbrandsen, C. Hyman, A.Y. Jeng, R.R. Neubig, and J. B. Cohen. 1981. Immunofluorescence localization at the mammalian neuromuscular junction of the M 43,000 protein of Torjgedo postsynaptic membranes. Proc NatlT Acad. Sci. USA 78: 5230–5234.Google Scholar
  18. 18.
    Peng, H.B., and S.C. Froehner. 1985. Association of the postsynaptic 43K protein with newly formed acetylcholine receptor clusters in cultured muscle cells. J Cell BioiJL 100: 1698–1795.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • H. Benjamin Peng
    • 1
  1. 1.Department of AnatomyUniversity of Illinois at ChicagoUSA

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