Regulation of Choline Phosphorylation in Rat Striatum

  • R. R. Reinhardt
  • L. Wecker


In cholinergic nerve terminals, choline is an important precursor molecule for the synthesis of both acetylcholine (ACh) and phosphorylcholine (PCh) through reactions catalyzed by choline acetyltransferase (CAT) and choline kinase (CK), respectively. While studies have indicated that CAT exists in both soluble and membrane associated forms which may subserve different primary functions in the nerve terminal (Smith and Carroll, 1980; Benishin and Carroll, 1983), little similar information has been available for CK. This enzyme catalyzes the first and possibly rate-limiting step (infante, 1977) in the cytidine pathway (Kennedy and Weiss, 1956) which is the major pathway for the incorporation of choline into phosphatidylcholine in brain (Ansell and Spanner, 1968). Since choline serves as a substrate for both CAT and CK, it might be expected that the nerve terminal has regulatory mechanisms to channel choline through the pathway that requires the precursor most at any given instant. Indeed, an association between the acetylation and phosphorylation pathways has been suggested (Ansell and Spanner, 1970; Collier, 1970). Therefore, to further our understanding of the utilization of choline by the cholinergic neuron, we studied the properties of CK and the modulation of choline phosphorylation by endogenous agents.


Cholinergic Neuron Choline Uptake Total Enzyme Activity Choline Kinase Choline Transport 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • R. R. Reinhardt
    • 1
  • L. Wecker
    • 1
  1. 1.Department of PharmacologyLouisiana State University Medical CenterNew OrleansUSA

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