Acyl-CoA Elongation Systems in Allium porrum Microsomes
The synthesis of very long chain fatty acids (VLCFA) has been demonstrated in cell-free preparations fron higher plants1,2,3. The leek epidermal cell microscme system is the best documented4,5,6 and it is in this system that the existence of an endogenous precursor ATP-dependent elongation system was established4. The elongation of exogenous acyl-CoAs in the absence of ATP has also been demonstrated5 and it was shown that acyl-CoAs were the reaction products of the acyl-CoA elongating system6. TCA and cerulenin inhibitory effects and in direct evidence suggest the presence of distinct C18-CoA and C2O-CoA elongating systems. In order to test this hypothesis, the elongation activities were solubilized.
KeywordsAnion Exchange Anion Exchange Chromatography Plant Lipid Sucrose Density Gradient Centrifugation Enrich Protein
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