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Acyl-CoA Elongation Systems in Allium porrum Microsomes

  • René Lessire
  • Jean-Jacques Bessoule
  • Claude Cassagne

Abstract

The synthesis of very long chain fatty acids (VLCFA) has been demonstrated in cell-free preparations fron higher plants1,2,3. The leek epidermal cell microscme system is the best documented4,5,6 and it is in this system that the existence of an endogenous precursor ATP-dependent elongation system was established4. The elongation of exogenous acyl-CoAs in the absence of ATP has also been demonstrated5 and it was shown that acyl-CoAs were the reaction products of the acyl-CoA elongating system6. TCA and cerulenin inhibitory effects and in direct evidence suggest the presence of distinct C18-CoA and C2O-CoA elongating systems. In order to test this hypothesis, the elongation activities were solubilized.

Keywords

Anion Exchange Anion Exchange Chromatography Plant Lipid Sucrose Density Gradient Centrifugation Enrich Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Kolattukudy, P.E., and Buckner, J.S., Biochem. Biophys. Res. Commun. 46: 801, (1972).PubMedCrossRefGoogle Scholar
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    Lessire, R., Juguelin, H., Moreau, P., and Cassagne, C., Phytochem. 24: 1187, (1985).CrossRefGoogle Scholar
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    Lessire, R., Juguelin, H., Moreau, P., and Cassagne, C., Arch. Biochem. Biophys. 239: 260, (1985).PubMedCrossRefGoogle Scholar
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    Lessire, R., Bessoule, J.J., and Cassagne, C., FEBS Lett. 187: 314, (1985).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • René Lessire
    • 1
  • Jean-Jacques Bessoule
    • 1
  • Claude Cassagne
    • 1
  1. 1.Institut de Biochimie Cellulaire et NeurochimieCNRSBordeaux cedexFrance

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