Substrate Specificity of Plant Lipases
In plants, lipases (EC 126.96.36.199) are active in lipid-storing tissues in seeds during germination. These lipases catalyze the hydrolysis of storage triacylglycerols to fatty acids, which are converted to sugars to support the growth of the embryo. Seed lipases have not been studied intensively, and our knowledge of them lags behind that of the mammalian and microbial enzymes (1, 2, 3). A unique feature of seed lipases may be that the enzyme from a certain plant species is relatively specific on the characteristic storage triacylglycerols of the same species. Our experimental results show that this specificity is indeed species-dependent, and is not due solely to the physical characteristics of the specific triacylglycerol in the substrate micelles.
KeywordsErucic Acid Pancreatic Lipase Castor Bean Ricinoleic Acid Porcine Pancreatic Lipase
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