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Substrate Specificity of Plant Lipases

  • Y. Y. Lin
  • Charles Yi
  • Julie Olsen
  • Anthony H. C. Huang

Abstract

In plants, lipases (EC 3.1.1.3) are active in lipid-storing tissues in seeds during germination. These lipases catalyze the hydrolysis of storage triacylglycerols to fatty acids, which are converted to sugars to support the growth of the embryo. Seed lipases have not been studied intensively, and our knowledge of them lags behind that of the mammalian and microbial enzymes (1, 2, 3). A unique feature of seed lipases may be that the enzyme from a certain plant species is relatively specific on the characteristic storage triacylglycerols of the same species. Our experimental results show that this specificity is indeed species-dependent, and is not due solely to the physical characteristics of the specific triacylglycerol in the substrate micelles.

Keywords

Erucic Acid Pancreatic Lipase Castor Bean Ricinoleic Acid Porcine Pancreatic Lipase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Galliard T. (1980) in The Biochemistry of Plants (Stumpf PK and Conn EE, eds), Vol 4, Academic Press, New York, pp 85–116.Google Scholar
  2. 2.
    Huang AHC. (1984) in Lipases (Brockman HL and Borgstrom B, eds). Elsevier, Amsterdam, pp. 419–442.Google Scholar
  3. 3.
    Huang AHC. (1987) in The Biochemistry of Plants (Stumpf PK and Conn EE, eds), Vol 9, Academic Press, New York, (in press)Google Scholar
  4. 4.
    Lin YH, Huang AHC. (1983) Arch. Biochem. Biophys. 225: 360–369.PubMedCrossRefGoogle Scholar
  5. 5.
    Lin YH, Yu C., Huang AHC. (1986) Arch. Biochem. Biophys. 244: 346–356.PubMedCrossRefGoogle Scholar
  6. 6.
    Dixon M, Chen SHP. (1979) Anal. Biochem. 97: 403–409.CrossRefGoogle Scholar
  7. 7.
    Entressangles B, Pasero L, Savary P, Sarda L, Desnuelle P. (1961) Bull. Soc. Chim. Biol. 43: 581–591.PubMedGoogle Scholar
  8. 8.
    Brockerhoff H., Jensen RG. (1974) Lipolytic Enzymes, Academic Press, New York.Google Scholar
  9. 9.
    Ory RL, St. Angelo AJ, Altschul AM. (1962). J. Lipid Res. 3: 99–105.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Y. Y. Lin
    • 1
  • Charles Yi
    • 1
  • Julie Olsen
    • 1
  • Anthony H. C. Huang
    • 1
  1. 1.Biology DepartmentUniversity of South CarolinaColumbinUSA

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