Cell Walls and Secondary Products as Obstacles to Plant Enzyme Isolation: Problems and Solutions, Including a Simple Liquid-Nitrogen Homogenizer for Bulk Tissue Extraction
When we first undertook studies of monoterpene biosynthesis in peppermint (Mentha piperita), we and other plant biochemists were handicapped by inadequate methodology and by inadequate understanding of plants. Gas chromatography was not yet available. It was assumed that secondary metabolites were synthesized slowly and irreversibly, so tracer experiments were conducted over periods of days instead of minutes or hours. Finally, the unique problems of plant enzymology were not understood.
KeywordsMiddle Lamella Phenol Oxidase Phenol Adsorbent Catechol Oxidase Living Plant Cell
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