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Cell Walls and Secondary Products as Obstacles to Plant Enzyme Isolation: Problems and Solutions, Including a Simple Liquid-Nitrogen Homogenizer for Bulk Tissue Extraction

  • Richard P. Sandstrom
  • W. David Loomis

Abstract

When we first undertook studies of monoterpene biosynthesis in peppermint (Mentha piperita), we and other plant biochemists were handicapped by inadequate methodology and by inadequate understanding of plants. Gas chromatography was not yet available. It was assumed that secondary metabolites were synthesized slowly and irreversibly, so tracer experiments were conducted over periods of days instead of minutes or hours. Finally, the unique problems of plant enzymology were not understood.

Keywords

Middle Lamella Phenol Oxidase Phenol Adsorbent Catechol Oxidase Living Plant Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Richard P. Sandstrom
    • 1
  • W. David Loomis
    • 1
  1. 1.Department of Biochemistry and BiophysicsOregon State UniversityCorvallisUSA

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