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Lipid Metabolism in Potato Leaf Disks: Effect of Calmodulin Antagonists

  • George J. Piazza
  • Robert A. Moreau

Abstract

Dibucaine (nupercaine), a local anesthetic, was recently shown to alter the lipid composition of barley-root membranes (1). When excised roots were placed in dibucaine there was an increase in the proportions of palmitic, stearic, and oleic acids and a decrease in the proportions of linoleic and linolenic acids. We have recently shown that the rate of autolytic degradation of phospholipids in potato leaf homogenates is inhibited by calmodulin antagonists (which include dibuucaine) and stimulated by calmodulin (2). This study was undertaken in order to investigate the effect of dibucaine and other calmodulin antagonists on the polar lipid composition of potato leaves.

Keywords

Hydrolytic Rate Lipolytic Enzyme Barley Root Potato Leaf Potato Leave 
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References

  1. 1.
    P.C. Jackson and J.B. St. John, Anesthetics alter the lipid composition of barley-root membrane, Planta 162: 415 (1984).CrossRefGoogle Scholar
  2. 2.
    R.A. Moreau and T.S. Isett, Autolysis of membrane lipids in potato leaf homogenates: effects of calmodulin and calmodulin antagonists, Plant Sci. 40: 95 (1985).CrossRefGoogle Scholar
  3. 3.
    J.C. Dittmer and A.W. Wells, Quantitative and qualitative analysis of lipids and lipid components, in “Methods in Enzymology” S.P. Colowick and N.O. Kaplan, eds., Academic Press, New York (1969).Google Scholar
  4. 4.
    P.G. Roughan and R.D. Batt, Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipid (monogalactosyl and digalactosyl diglycerides) in plant tissues, Anal. Biochem., 22: 74 (1968).PubMedCrossRefGoogle Scholar
  5. 5.
    Y.Y. Leshem, S. Sridhara, and J.E. Thompson, Involvement of calcium and calmodulin in membrane deterioration during senescence of pea foliage, Plant Physiol. 75: 329 (1984).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • George J. Piazza
    • 1
  • Robert A. Moreau
    • 1
  1. 1.Agricultural Research ServiceU. S. Department of AgriculturePhiladelphiaUSA

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