On the Synthesis of Digalactosyldiacylglycerol in Chloroplasts, and Its Relation to Monogalactolipid Synthesis

  • Jef F. G. M. Wintermans
  • Johan W. M. Heemskerk


About a quarter century after the first observation that galactolipids are specific membrane lipids of chloroplasts (1), it seems appropriate to make a short survey of the knowledge gained about their biosynthesis. In contrast to other papers (2–5) we shall emphasize on the synthesis of digalactosyldiacylglycerol (DGDG*), which has been a subject of much confusion.


Spinach Chloroplast Envelope Membrane Euglena Gracilis Chloroplast Envelope Intact Chloroplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



galactolipid: galactolipid acyltransferase


galactolipid: galactolipid galactosyltransferase


mono-, di-, tri-, and tetragalactosyldiacylglycerol

PC and PG

phosphatidylcholine and -glycerol


UDPGal: diacylglycerol galactosyltransferase.


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  1. 1.
    Wintermans, J.F.G.M. (1960) Biochim. Biophys. Acta 44, 49–54.PubMedCrossRefGoogle Scholar
  2. 2.
    Heinz, E. (1977) In Lipids and Lipid Polymers in Higher Plants (Tevini, M. and Lichtenthaler, H.K., eds.) pp. 102–120, Springer Verlag Berlin.CrossRefGoogle Scholar
  3. 3.
    Douce, R. and Joyard, J. (1980) In The Biochemistry of Plants, Vol. 4, Lipids: Structure and function (Stumpf, P.K., ed.) pp. 321–362, Academic Press, New York.Google Scholar
  4. 4.
    Roughan, P.G. and Slack, C.R. (1982) Annu. Rev. Plant Physiol. 33, 97–132.CrossRefGoogle Scholar
  5. 5.
    Douce, R., Block, M.A., Dorne, A.-J. and Joyard, J. (1984) In Subcellular Biochemistry, Vol. 10 (Roodyn, D.B., ed.) pp. 1–84, Plenum Press, New York.CrossRefGoogle Scholar
  6. 6.
    Douce, R., Holtz, R.B. and Benson, A.A. (1973) J. Biol. Chem. 248, 7215–7222.PubMedGoogle Scholar
  7. 7.
    Douce, R. (1974) Science 183, 852–853.PubMedCrossRefGoogle Scholar
  8. 8.
    Joyard, J. and Douce, R. (1977) Biochim. Biophys. Acta 486, 273–285.PubMedGoogle Scholar
  9. 9.
    Joyard, J. and Douce, R. (1976) Biochim. Biophys. Acta 424, 125–131.PubMedGoogle Scholar
  10. 10.
    Frentzen, M., Heinz, E., McKeon, T.A. and Stumpf,. P.K. (1983) Eur. J. Biochem. 129, 629–636.PubMedCrossRefGoogle Scholar
  11. 11.
    Ongun, A. and Mudd, J.B. (1968) J. Biol. Chem. 243, 1558–1566.PubMedGoogle Scholar
  12. 12.
    Siebertz, M. and Heinz, E. (1977) Hoppe Seyler’s Z. Physiol. Chem. 358, 27–34.PubMedCrossRefGoogle Scholar
  13. 13.
    Van Besouw, A. and Wintermans, J.F.G.M. (1978) Biochim. Biophys. Acta 529, 44–53.PubMedGoogle Scholar
  14. 14.
    Heemskerk, J.W.M., Bögemann, G. and Wintermans, J.F.G.M. (1983) Biochim.Biophys. Acta 754, 181–189.Google Scholar
  15. 15.
    Siebertz, H.P., Heinz, E., Linscheid, M., Joyard, J. and Douce, R. (1979) Eur. J. Biochem. 101, 429–438.PubMedCrossRefGoogle Scholar
  16. 16.
    Wintermans, J.F.G.M., Van Besouw, A. and Bögemann, G. (1981) Biochim. Biophys. Acta 663, 99–107.PubMedGoogle Scholar
  17. 17.
    Dome, A.-J., Block, M.A., Joyard, J. and Douce, R. (1982) FEBS Lett. 145, 30–34.CrossRefGoogle Scholar
  18. 18.
    Heemskerk, J.W.M., Bögemann, G., Scheijen, M.A.M. and Wintermans, J.F.G.M. (1986) Anal. Biochem. 154, 88–92.CrossRefGoogle Scholar
  19. 19.
    Heemskerk, J.W.M. (1986) Ph. D. Thesis, University of Nijmegen, Nijmegen (The Netherlands).Google Scholar
  20. 20.
    Heemskerk, J.W.M., Scheijen, M.A.M., Jacobs, F.H.H. and Wintermans, J.F.G.M. (This volume).Google Scholar
  21. 21.
    Heinz. E., Bertrams, M., Joyard, J. and Douce, R. (1978) Z. Pflanzenphysiol. 87, 325–331.Google Scholar
  22. 22.
    Heinz, E. (1973) Z. Pflanzenphysiol. 69, 359–376.Google Scholar
  23. 23.
    Block, M.A., Dorne, A.-J., Joyard, J. and Douce, R. (1983) J. Biol. Chem. 258, 13281–13286.PubMedGoogle Scholar
  24. 24.
    Heemskerk, J.W.M., Bögemann, G. and Wintermans, J.F.G.M. (1985) Biochim. Biophys. Acta 835, 212–220.Google Scholar
  25. 25.
    Cline, K. and Keegstra, K. (1983) Plant Physiol. 71, 366–372.PubMedCrossRefGoogle Scholar
  26. 26.
    Heemskerk, J.W.M., Wintermans, J.F.G.M., Joyard, J., Block, M.A., Dorne, A.-J. and Douce, R. (1986) Biochim. Biophys. Acta 877, 281–289.Google Scholar
  27. 27.
    Heemskerk, J.W.M., Jacobs, F.H.H., Bögemann, G. and Wintermans, J.F.G.M. (This volume).Google Scholar
  28. 28.
    Van Besouw, A. and Wintermans, J.F.G.M. (1979) FEBS Lett. 102, 33–37.PubMedCrossRefGoogle Scholar
  29. 29.
    Douce, R. and Guillot-Salomon, T. (1970) FEBS Lett. 11, 121–124.PubMedCrossRefGoogle Scholar
  30. 30.
    McKee, J.W.A. and Hawke, J.C. (1979) Arch. Biochim. Biophys. 197, 322–332.CrossRefGoogle Scholar
  31. 31.
    Renkonen, O. and Bloch, K. (1969) J. Biol. Chem. 244, 4899–4903.PubMedGoogle Scholar
  32. 32.
    Wintermans, J.F.G.M., Van Besouw, A., Bögemann, G. and Aerts, J. (1980) In Biogenesis and Function of Plant Lipids (Mazliak, P., Benveniste, P., Costes, C. and Douce, R., eds.) pp. 49–56, Elsevier, Amsterdam.Google Scholar
  33. 33.
    Blee, E. and Schantz, R. (1978) Plant Sci. Lett. 13, 247–255.CrossRefGoogle Scholar
  34. 34.
    Mendiola-Morgenthaler, L., Eichenberger, W. and Boschetti, A. (1985) Plant Sci. 41, 97–104.CrossRefGoogle Scholar
  35. 35.
    Liedvogel, B. and Kleinig, H. (1977) Planta 133, 249–253.CrossRefGoogle Scholar
  36. 36.
    Williams, J.P., Khan, M. and Leung, S. (1975) J. Lipid. Res. 16, 61–65.PubMedGoogle Scholar
  37. 37.
    Siebertz, H.P., Heinz, E., Joyard, J. and Douce, R. (1980) Eur. J. Biochem. 108, 177–185.PubMedCrossRefGoogle Scholar
  38. 38.
    Williams, J.P. and Khan, M.U. (1982) Biochim. Biophys. Acta 713, 177–184.Google Scholar
  39. 39.
    Sato, N. and Murata, N. (1982) Biochim. Biophys. Acta 710, 271–278.Google Scholar
  40. 40.
    Sato, N. and Murata, N. (1982) Plant Cell Physiol. 23, 1115–1120.Google Scholar
  41. 41.
    Gounaris, K., Sen, A., Brain, A.P.R., Quinn, P.J. and Williams, W.P. (1983) Biochim. Biophys. Acta 728, 129–139.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Jef F. G. M. Wintermans
    • 1
  • Johan W. M. Heemskerk
    • 1
  1. 1.Botanisch LaboratoriumUniversity of Nijmegen, ToernooiveldNijmegenThe Netherlands

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