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Plasma Membrane and Tonoplast Fractions Isolated from Spinach Leaves by Preparative Free Flow Electrophoresis: Effect of Photoinduction

  • C. Penel
  • G. Auderset
  • S. Kiefer
  • A. Sandelius
  • A. Brightman
  • H. Greppin
  • D. James Morré

Abstract

A change in membrane structure as a result of photoinduction of the flowering response in plants was predicted from physiological studies (Penel and Greppin, 1974; Lenk et al., 1981; Karege et al., 1982). With the availability of purified fractions of plasma membrane and tonoplast from green leaves (Auderset et al., in press), a study was initiated to examine these membranes for structural and compositional changes associated with photoinduction. Spinach (Spinach oleracea) was investigated as an example of a species induced to flower by continuous light (so-called long-day plant) and Japanese morning glory (Pharbitis nil) as an example of a plant photoinduced by a long night (so-called short-day plant).

Keywords

Membrane Thickness Floral Induction Plasma Membrane Vesicle Spinach Leave Thin Layer Chroma 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Auderset, G., Sandelius, A. S., Penel, C., Brightman, A., Greppin, H., and Morré, D. J., Isolation of plasma membrane and tonoplast fractions from spinach leaves by preparative free-flow electrophoresis and effect of photoinduction, Physiol. Plant, (in press).Google Scholar
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • C. Penel
    • 1
  • G. Auderset
    • 1
  • S. Kiefer
    • 1
  • A. Sandelius
    • 2
  • A. Brightman
    • 3
  • H. Greppin
    • 1
  • D. James Morré
    • 3
  1. 1.University of GenevaSwitzerland
  2. 2.University of GöteborgSweden
  3. 3.Purdue UniversityWest LafayetteUSA

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