Plasma Membrane and Tonoplast Fractions Isolated from Spinach Leaves by Preparative Free Flow Electrophoresis: Effect of Photoinduction
A change in membrane structure as a result of photoinduction of the flowering response in plants was predicted from physiological studies (Penel and Greppin, 1974; Lenk et al., 1981; Karege et al., 1982). With the availability of purified fractions of plasma membrane and tonoplast from green leaves (Auderset et al., in press), a study was initiated to examine these membranes for structural and compositional changes associated with photoinduction. Spinach (Spinach oleracea) was investigated as an example of a species induced to flower by continuous light (so-called long-day plant) and Japanese morning glory (Pharbitis nil) as an example of a plant photoinduced by a long night (so-called short-day plant).
KeywordsMembrane Thickness Floral Induction Plasma Membrane Vesicle Spinach Leave Thin Layer Chroma
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