Evidences for Different Acyl Lipid Domains in Spinach and Oat Thylakoid Membranes Supporting Various Photosynthetic Functions

  • Paul-André Siegenthaler
  • Christian Giroud
  • Jana Smutny


One approach to studying the role of acyl lipids in thylakoid membranes has been to incubate intact thylakoids with lipolytic enzymes and then to determine the relationships between the changes occuring in their lipid content and composition and, simultaneously, in their photochemical activities1–3. whilst phospholipases hydrolyze specifically the two phospholipids (PG and PC) encountered in the thylakoid membrane, lipolytic acyl hydrolases deacylate not only glycolipids (MGDG, DGDG, SQDG) but also phospholipids. The non-specificity of the enzymes used does not allow an unequivocal assessement of function to each of these lipids4. Moreover, in most studies, hydrolysis of acyl lipids have been carried out under conditions allowing the attack of all acyl lipids, i.e. of lipids which are localized indistinctly in both monolayers of the membrane. However, it is now well established that all acyl lipids of the thylakoid membrane present a transversal asymmetric distribution1,3–8, suggesting that different acyl lipid domains may sustain different functions1,3. Thus, it would be of great interest to establish relationships between the hydrolysis of specific acyl lipids in the outer membrane monolayer only and the resulting changes occurring in various electron flow activities.


Thylakoid Membrane Lipolytic Enzyme Acyl Lipid Phospholipid Molecule Photosynthetic Function 


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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Paul-André Siegenthaler
    • 1
  • Christian Giroud
    • 1
  • Jana Smutny
    • 1
  1. 1.Laboratoire de Physiologie végétaleUniversité de NeuchâtelNeuchâtelSwitzerland

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