Triterpenoid Biosynthesis in EuphorbiaLathyris
Triterpenols and their esters make up 4–5% of the dry weight of Euphorbia lathyris.1 A significant amount of these triterpenoids are synthesized and stored within laticifer cells where these compounds constitute up to 50% of the dry weight of the latex.2 We are interested in the nature of these compounds and the organization and regulation of their biosynthesis. Here we report on recent work investigating the control of carbon allocation to these compounds, further elucidation of the identity of the triterpenols found in latex, evidence indicating that the conversion of HMG-CoA to meva-lonate (MVA) is rate-limiting in the biosynthesis of triterpenoids in latex, and steps in the purification of HMG-CoA reductase (HMGR).
KeywordsCarbon Allocation Terpenoid Biosynthesis Glucose Incorporation HMGR Activity Organic Acid Column
Unable to display preview. Download preview PDF.
- 3.D. H. R. Barton, D. M. Harrison, G. P. Moss, and D. A. Widdowson, Investigations on the biosynthesis of steroids and triterpenoids, J. Chem. Soc. (C), 775 (1970).Google Scholar
- 6.C.C. Black, D. A. Smyth, and M-X Wu, Pyrophosphate-dependent glycolysis and regulation by fructose 2, 6-bisphosphate in plants, in “Nitrogen Fixation and CO2 Metabolism,” P. W. Ludden and J. E. Burris, eds., Elsevier Sci. Pub. Co., New York (1985).Google Scholar