Advertisement

Triterpenoid Biosynthesis in EuphorbiaLathyris

  • C. L. Skrukrud
  • S. E. Taylor
  • D. R. Hawkins
  • M. Calvin

Abstract

Triterpenols and their esters make up 4–5% of the dry weight of Euphorbia lathyris.1 A significant amount of these triterpenoids are synthesized and stored within laticifer cells where these compounds constitute up to 50% of the dry weight of the latex.2 We are interested in the nature of these compounds and the organization and regulation of their biosynthesis. Here we report on recent work investigating the control of carbon allocation to these compounds, further elucidation of the identity of the triterpenols found in latex, evidence indicating that the conversion of HMG-CoA to meva-lonate (MVA) is rate-limiting in the biosynthesis of triterpenoids in latex, and steps in the purification of HMG-CoA reductase (HMGR).

Keywords

Carbon Allocation Terpenoid Biosynthesis Glucose Incorporation HMGR Activity Organic Acid Column 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    E. K. Nemethy, J. W. Otvos, and M. Calvin, Analysis of extractables from one Euphorbia, J. Am. Oil Chem Soc. 56: 957 (1979).CrossRefGoogle Scholar
  2. 2.
    E. K. Nemethy, C. Skrukrud, G. J. Piazza, and M. Calvin, Terpenoid biosynthesis in Euphorbia latex, Biochim. Biophys. Acta 760: 343 (1983).CrossRefGoogle Scholar
  3. 3.
    D. H. R. Barton, D. M. Harrison, G. P. Moss, and D. A. Widdowson, Investigations on the biosynthesis of steroids and triterpenoids, J. Chem. Soc. (C), 775 (1970).Google Scholar
  4. 4.
    M. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72: 248 (1976).PubMedCrossRefGoogle Scholar
  5. 5.
    P. E. Nielsen, H. Nishimura, J. W. Otvos, and M. Calvin, Plant crops as a source of fuel and hydrocarbon-like materials, Science 198: 942 (1977).PubMedCrossRefGoogle Scholar
  6. 6.
    C.C. Black, D. A. Smyth, and M-X Wu, Pyrophosphate-dependent glycolysis and regulation by fructose 2, 6-bisphosphate in plants, in “Nitrogen Fixation and CO2 Metabolism,” P. W. Ludden and J. E. Burris, eds., Elsevier Sci. Pub. Co., New York (1985).Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • C. L. Skrukrud
    • 1
  • S. E. Taylor
    • 1
  • D. R. Hawkins
    • 1
  • M. Calvin
    • 1
  1. 1.Lawrence Berkeley Laboratory and Department of ChemistryUniversity of CaliforniaBerkeleyUSA

Personalised recommendations