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Regulation of Terpenoid Biosynthesis in Tapped Latex

  • George J. Piazza
  • Edward J. Saggese
  • Marvin P. Thompson

Abstract

The tapped latex of Euphorbia lathyris can convert acetate to several structurally different triterpenols (TOH) and their fatty acid esters (TE) (1). Simple low speed centrifugation of the latex affords a pellet that utilizes mevalonic acid as a triterpenoid precursor (2). Biosynthetic activity in the latex pellet is absolutely dependent upon the presence of an osmoticum (0.4 M sorbitol), indicating that biosynthesis occurs in an osmotically sensitive organelle. Calcium constitutes about 7.5% of dry latex weight, and the addition of Ca2+ to an EGTA-treated latex pellet stimulates triterpene biosynthesis. This study was undertaken to investigate whether the calcium binding protein, calmodulin, mediates Ca2+ effects upon triterpenoid biosynthesis.

Keywords

Fatty Acid Ester Calcium Binding Protein Mevalonic Acid Bovine Heart Phosphodiesterase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    G.J. Piazza, Calmodulin in plants, in: “Calcium Binding Proteins,” M.P. Thompson, ed., CRC Press, Boca Raton, FL., in press.Google Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • George J. Piazza
    • 1
  • Edward J. Saggese
    • 1
  • Marvin P. Thompson
    • 1
  1. 1.Agricultural Research ServiceU. S. Department of AgriculturePhiladelphiaUSA

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