Inhibition of Growth of Celery Cells by Paclobutrazol and Its Reversal by Added Sterols

  • Penny A. Haughan
  • John R. Lenton
  • L. John Goad


Paclobutrazol, (2RS, 3RS)-1-(4-chlorophenyl)-4, 4-dimethyl-2-(1, 2, 4-triazol-1-yl) pentan-3-ol, is a synthetic plant growth retardant 1, 2 which also has a high fungicidal activity.3, 4 The antifungal activity of paclobutrazol is related to its inhibition of sterol biosynthesis4 by interaction with the cytochrome P-450 system required for 14-demethylation of sterol precursors.3, 5 This results in the accumulation of 14 α - methylsterols in fungi.4 Paclobutrazol also exerts plant growth regulatory effects by interfering with gibberellin biosynthesis at the step which is catalysed by the cytochrome P-450 requiring enzyme, kaurene oxidase.2, 3, 6


Growth Retardant Sterol Biosynthesis Tobacco Seedling Gibberellin Biosynthesis Cell Culture Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    B.G. Lever, S.J. Shearing and J.J. Batch, PP333-A new broad spectrum growth retardant. Proc. Br. Crop Protect. Conf. Pests Dis. 1: 3 (1982)Google Scholar
  2. 2.
    S.Y. Wang, J.K. Byun and G.L. Steffens, Controlling plant growth via the gibberellin biosynthesis system — II. Biochemical and physiological alterations in apple seedings. Physiol. Plant 63: 169 (1985)CrossRefGoogle Scholar
  3. 3.
    B. Sugavanam, Diastereoisomers and enantiomers of paclobutrazol: their preparation and biological activity. Pestic. Sci. 15: 296 (1984)CrossRefGoogle Scholar
  4. 4.
    B.C. Baldwin and T.E. Wiggins, Action of fungicidal triazoles of the diclobutrazol series on Ustilago maydis. Pestic. Sci. 15: 156 (1984)CrossRefGoogle Scholar
  5. 5.
    T.E. Wiggins and B.C. Baldwin, Binding of azole fungicides related to diclobutrazol to cytochrome P-450. Pestic. Sci. 15: 206 (1984)CrossRefGoogle Scholar
  6. 6.
    J. Dalziel and D.K. Lawrence, Biochemical and biological effects of kauarene oxidase inhibitors, such as paclobutrazol. Monograph of the British Plant Growth Regulator Group No 11, p.43 (1984)Google Scholar
  7. 7.
    H. Buchenauer and E. Röhner, Effect of triadimefon and triadimenol on growth of various plant species as well as gibberellin content and sterol metabolism in shoots of barley seedlings. Pest. Biochem. Physiol. 15: 58 (1981)CrossRefGoogle Scholar
  8. 8.
    T.J. Douglas and L.G. Paleg, Inhibition of sterol biosynthesis and stem elongation of tobacco seedlings induced by some hypocholesterolemic agents. J. Exptl. Botany 32: 59 (1981).CrossRefGoogle Scholar
  9. 9.
    P. Schmitt, A. Rahier and P. Benveniste, Inhibition of sterol biosynthesis in suspension cultures of bramble cells. Physiol. Veg. 20: 559 (1982)Google Scholar
  10. 10.a)
    K. Nitsche, K. Grossmann, E. Sauerbrey and J. Jung, Influence of the growth retardant tetcyclacis on cell division and cell elongation in plants and cell cultures of sunflower, soybean and maize. J Plant Physiol. 118: 209 (1985).CrossRefGoogle Scholar
  11. b).
    K. Grossmann, W. Rademacher and J. Jung, Effects of NDA, a new plant growth retardant, on cell culture growth of Zea mays. J. Plant Growth Regul. 2: 19 (1983).CrossRefGoogle Scholar
  12. c).
    K. Grossmann, E.W. Weiler and J. Jung, Effects of different sterols on the inhibition of cell culture growth by the growth retardant tetcyclacis. Planta 164: 370 (1985)CrossRefGoogle Scholar
  13. 11.
    H.D. Sisler, N.N. Ragsdale and W.F. Waterfield, Biochemical aspects of the fungitoxic and growth regulatory action of fenarimol and other pyrimidin-5-ylmethanols. Pestic Sci. 15: 167 (1984)CrossRefGoogle Scholar
  14. 12.
    R.J. Rodriguez, C. Low, C.D.K. Bottema and L.W. Parks, Multiple functions of sterols in Saccharomyces cerevisiae. Biochim. Biophys. Acta 837: 336 (1985)PubMedGoogle Scholar
  15. 13.
    W.D. Nes and R.C. Heupel, Physiological requirement for biosynthesis of multiple 24 β-methyl sterols in Gibberella fujikuroi. Arch. Biochem. Biophys. 244: 211 (1986)PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Penny A. Haughan
    • 1
  • John R. Lenton
    • 2
  • L. John Goad
    • 1
  1. 1.Department of BiochemistryUniversity of LiverpoolLiverpoolUK
  2. 2.Long Ashton Research StationLong Ashton, BristolUK

Personalised recommendations