Complete Amino Acid Sequence of Non-specific Lipid Transfer Protein from Castor Bean Seeds
A lipid transfer protein (LTP) was purified from germinated castor bean seeds to homogeneity by column chromatographies. This protein, possessing about 9, 000 of the molecular weight and higher than 10;5 of the isoelectric point, transferred PC, PI, PE, PG, PA, MGDG and DGDG, but not DG and TG, and the transfer activity remained unchanged after 30 min-heating at 90°C. This protein is one of non-specific LTPs as isolated from maize seedlings1 and spinach leaves2. This paper presents the determination of complete amino acid sequence of castor bean non-specific LTP. Based on the amino acid sequence, structural aspect is discussed in relation to the interaction of the protein with the lipid molecule.
KeywordsIsoelectric Point Lipid Molecule Castor Bean Transfer Activity Bovine Liver
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