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Complete Amino Acid Sequence of Non-specific Lipid Transfer Protein from Castor Bean Seeds

  • Mitsuhiro Yamada
  • Shinichiro Watanabe
  • Kunio Takishima
  • Gunji Mamiya

Abstract

A lipid transfer protein (LTP) was purified from germinated castor bean seeds to homogeneity by column chromatographies. This protein, possessing about 9, 000 of the molecular weight and higher than 10;5 of the isoelectric point, transferred PC, PI, PE, PG, PA, MGDG and DGDG, but not DG and TG, and the transfer activity remained unchanged after 30 min-heating at 90°C. This protein is one of non-specific LTPs as isolated from maize seedlings1 and spinach leaves2. This paper presents the determination of complete amino acid sequence of castor bean non-specific LTP. Based on the amino acid sequence, structural aspect is discussed in relation to the interaction of the protein with the lipid molecule.

Keywords

Isoelectric Point Lipid Molecule Castor Bean Transfer Activity Bovine Liver 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    D. Douady, M. Grosbois, F. Guerbette and J.C. Kader, Biochim. Biophys. Acta 710: 143 (1982)Google Scholar
  2. 2.
    J.C. Kader, M. Julienne and C. Vergnolle, Eur. J. Biochem. 139: 411 (1984)PubMedCrossRefGoogle Scholar
  3. 3.
    S. Watanabe and M. Yamada, Biochim. Biophys. Acta 876: 116 (1986)Google Scholar
  4. 4.
    K. Takishima, S. Watanabe, M. Yamada and G. Mamiya, Biochim. Biophys. Acta 870: 248 (1986)CrossRefGoogle Scholar
  5. 5.
    P. Bouillon, C. Drischel, C. Vergnolle, J.C. Kader and H. Duranton, C.R. Acad. Sci. Paris, t. 300, Serie III, No 10, Physiol. Veg. 421 (1985).Google Scholar
  6. 6.
    R. Akeroyd, P. Moonen, J. Westerman, W. C. Puyk and K. W. A. Wirtz, Eur. J. Biochem. 114: 385 (1981)PubMedCrossRefGoogle Scholar
  7. 7.
    P. Y. Chou and G. D. Fasman, Adv. Enzymol. 47: 45 (1978)PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Mitsuhiro Yamada
    • 1
  • Shinichiro Watanabe
    • 1
  • Kunio Takishima
    • 2
  • Gunji Mamiya
    • 2
  1. 1.Department of BiologyThe University of TokyoTokyo 153Japan
  2. 2.Department of BiochemistryNational Defense Medical CollegeSaitama 359Japan

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