Effect of Dilution on FT3 and FT4 Concentration in Serum Containing Substances That Interact with Thyroid Hormone-Binding Proteins
Almost all available methods for FT3 and FT4 determination include a dilution step of serum. Even in the direct determination using dialysis, a method that has been regarded as a reference technique, dilution is unavoidable. The effect of dilution will be more pronounced on an interacting but weakly bound substance than on the binding of thyroid hormones to the transport proteins. This means that, in serum that contains such compounds, the free concentration of thyroid hormones will be underestimated, if dilution is employed.
KeywordsThyroid Hormone Equilibrium Dialysis Dialysis Tube Free Thyroid Hormone Thyroid Hormone Status
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