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Measurement of “Free Thyroxine” in Dried Blood Samples on Filter Paper by Radio- and Enzyme-Immunoassay for Monitoring Thyroid Function

  • Kiyoshi Miyai
  • Naoshige Hata
  • Hitoshi Mizuta
  • Yuichi Endo
  • Masao Ito
  • Osamu Nose

Abstract

Mass screening for congenital hypothyroidism in newborns has been developed because the irreversible mental retardation caused by this disorder can be prevented by early treatment. These programs include measurement of thyrotropin or total thyroxine (T4), or both, in dried blood samples. Measurement of total T4 is useful for detecting primary, secondary, and tertiary hypothyroidism, but gives false-positive results for subjects with abnormal concentrations of thyroxine-binding globulin (TBG), who do not need to be treated. Hence, we developed an enzyme immunoassay (EIA) and a radioimmunoassay (RIA) for measuring free T4 (FT4) in dried blood samples on filter paper.

Keywords

Congenital Hypothyroidism Sodium Carbonate Solution Total Thyroxine Test Blood Spot Tertiary Hypothyroidism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Ito M, Miyai K, Doi K, et al. Clin Chem 30: 1682, 1984.PubMedGoogle Scholar
  2. 2.
    Amino N, Nishi K, Nakatani K, et al. Clin Chem 29: 321, 1983.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Kiyoshi Miyai
    • 1
  • Naoshige Hata
    • 1
  • Hitoshi Mizuta
    • 1
  • Yuichi Endo
    • 1
  • Masao Ito
    • 1
  • Osamu Nose
    • 1
  1. 1.Department of Laboratory Medicine, Department of PediatricsOsaka University Medical SchoolOsakaJapan

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