Human Hepatoma (Hep G2) Cells Secrete a Novel T4-Binding Protein (27 K Protein)
TBG, prealbumin, and albumin represent the major thyroid hormone transport proteins in the blood (1), but other proteins interact with thyroid hormones, although their physiological role is still unclear (2). We have recently reported purification of a novel T4-binding protein from human serum, called 27 K protein for its apparent molecular weight in SDS-PAGE (3). In the present paper we show that 27 K protein is synthesized and secreted by human hepatoma (Hep G2) cells, and partially characterize it both in whole-cell and cell-free systems.
KeywordsHuman Hepatoma Xenopus Laevis Oocyte Rabbit Reticulocyte Lysate Sucrose Gradient Centrifugation Discontinuous Sucrose Gradient
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