Human Hepatoma (Hep G2) Cells Secrete a Novel T4-Binding Protein (27 K Protein)

  • Luigi Bartalena
  • Settimio Grimaldi
  • Jacob Robbins


TBG, prealbumin, and albumin represent the major thyroid hormone transport proteins in the blood (1), but other proteins interact with thyroid hormones, although their physiological role is still unclear (2). We have recently reported purification of a novel T4-binding protein from human serum, called 27 K protein for its apparent molecular weight in SDS-PAGE (3). In the present paper we show that 27 K protein is synthesized and secreted by human hepatoma (Hep G2) cells, and partially characterize it both in whole-cell and cell-free systems.


Human Hepatoma Xenopus Laevis Oocyte Rabbit Reticulocyte Lysate Sucrose Gradient Centrifugation Discontinuous Sucrose Gradient 
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  1. 1.
    Robbins J and Bartalena L. In G Hennemann (ed), Thyroid Hormone Metabolism, Marcel Dekker, 1985, in press.Google Scholar
  2. 2.
    Robbins J, Cheng S-Y, Gershengorn MC, et al. Recent Prog Horm Res 34: 477, 1978.PubMedGoogle Scholar
  3. 3.
    Grimaldi S, Bartalena L, and Robbins J. Ann Endocrinol (Paris) 44: Abstr. 113, 1983.Google Scholar
  4. 4.
    Bartalena L, Tata JR, and Robbins J. J Biol Chem 259: 13605, 1984.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Luigi Bartalena
    • 1
    • 3
  • Settimio Grimaldi
    • 2
    • 3
  • Jacob Robbins
    • 3
  1. 1.Cattedra di Endocrinologia e Medicina CostituzionaleUniversity of PisaPisaItaly
  2. 2.Instituto di Medicina Sperimentale del C.N.R.University of RomeRomeItaly
  3. 3.Clinical Endocrinology BranchNIADDK, NIHBethesdaUSA

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