Thyroid Hormone Metabolism in Kidney Epithelial Cells in Continuous Culture
Renal T4 to T3 conversion is catalyzed by membrane-bound enzyme, iodothyronine 5′deiodinase (5′D), that prefers rT3 as a substrate, is inhibited by propylthiouracil (PTU), and shows ping-pong reaction kinetics with thiols as cosubstrate (1–3). Recent studies have shown that >90% of the kidney 5′D is associated with the tubular epithelium of the outer cortex (4). Since established renal epithelial cell lines that retain properties of either the proximal or the distal convoluted tubule are available, selected cell lines were examined for the presence of 5′D activity and for the presence of nuclear T3 receptors.
KeywordsThyroid Hormone MDCK Cell Renal Epithelial Cell Tubular Epithelium Distal Convoluted Tubule
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