Advertisement

Thyroid Hormone Metabolism in Kidney Epithelial Cells in Continuous Culture

  • Jack L. Leonard

Abstract

Renal T4 to T3 conversion is catalyzed by membrane-bound enzyme, iodothyronine 5′deiodinase (5′D), that prefers rT3 as a substrate, is inhibited by propylthiouracil (PTU), and shows ping-pong reaction kinetics with thiols as cosubstrate (1–3). Recent studies have shown that >90% of the kidney 5′D is associated with the tubular epithelium of the outer cortex (4). Since established renal epithelial cell lines that retain properties of either the proximal or the distal convoluted tubule are available, selected cell lines were examined for the presence of 5′D activity and for the presence of nuclear T3 receptors.

Keywords

Thyroid Hormone MDCK Cell Renal Epithelial Cell Tubular Epithelium Distal Convoluted Tubule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Leonard JL and Rosenberg IN. Endocrinology 107: 1376, 1980.PubMedCrossRefGoogle Scholar
  2. 2.
    Leonard JL and Rosenberg IN. Endocrinology 103: 2137, 1978.PubMedCrossRefGoogle Scholar
  3. 3.
    Leonard JL and Rosenberg IN. Biochim Biophys Acta 659: 205, 1981.PubMedCrossRefGoogle Scholar
  4. 4.
    Leonard JL, Frank SJ, and Gogolin PJ. 60th Amer Thyroid Assoc Meeting, NY, T-43, 1984.Google Scholar
  5. 5.
    Weeke J and Orshov H. Scand J Clin Lab Invest 32: 357, 1973.PubMedCrossRefGoogle Scholar
  6. 6.
    Bellabarba D, Petersen RE, and Sterling K. J Clin Endocrinol Metab 28: 305, 1968.PubMedCrossRefGoogle Scholar
  7. 7.
    Kolodny JM, Leonard JL, Larsen PR, et al. Endocrinology 117: 1848, 1985.PubMedCrossRefGoogle Scholar
  8. 8.
    Samuels HH and Tsai JS. Proc Natl Acad Sci USA 70: 3488, 1973.PubMedCrossRefGoogle Scholar
  9. 9.
    Scatchard G. Ann NY Acad Sci 51: 660, 1979.CrossRefGoogle Scholar
  10. 10.
    Perantoni A and Berman JJ. In Vitro 15: 446, 1979.PubMedCrossRefGoogle Scholar
  11. 11.
    Sanders MJ and Misfeldt DS. Fed Proc 39: 736, 1980.Google Scholar
  12. 12.
    Handler JS, Perkins FM, and Johnson JP. Am J Physiol 238: F1, 1980.PubMedGoogle Scholar
  13. 13.
    Teitelbaum AP and Strewler GJ. Endocrinology 114: 980, 1984.PubMedCrossRefGoogle Scholar
  14. 14.
    Rindler MJ, Taub M, and Saier Jr MH. J Biol Chem 254: 1 1431, 1979.Google Scholar
  15. 15.
    Misfeldt DS, Hammammoto ST, and Pitelka DR. Proc Natl Acad Sci USA 73: 1212, 1976.PubMedCrossRefGoogle Scholar
  16. 16.
    Leonard JL and Rosenberg IN. Endocrinology 103: 274, 1978.PubMedCrossRefGoogle Scholar
  17. 17.
    Savouret J-F, Eberhardt NL, Cathala G, et al. Endocrinology 116: 1259, 1985.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Jack L. Leonard
    • 1
  1. 1.Department of MedicineBrigham and Women’s Hospital and Harvard Medical SchoolBostonUSA

Personalised recommendations