Production of Both Types of Insulin-Like Growth Factors by Primary Cultures of Ovine Thyroid Cells

  • William W. Mak
  • Banani Bhaumick
  • R. Marvin Bala
  • Jeffrey E. Kudlow
  • Gerard N. Burrow


One of the major tenets of thyroidology concerns TSH control of thyroid gland growth (1). The suppression of goiter by thyroid hormone is based on this premise. However, the effect of TSH on growth has been variable in different thyroid cell cultures in vitro. Recent advances in cell culture technique have allowed the culturing of ovine thyroid cells in chemically-defined, serum-free medium and, thus, the study on the role of TSH on thyroid growth and differentiation in the absence of serum factors. In primary cultures of ovine thyroid cells, TSH stimulates a number of differentiated aspects of thyroid function including follicle formation, iodide uptake and organification, cAMP production, thyroglobulin, thyroxine and triiodothyronine synthesis (2), as well as production of plasminogen activator (3). But, TSH does not stimulate growth in these ovine cells. The possibility existed that TSH did not stimulate growth directly. Conceivably, thyroid cells were producing growth factors as an aspect of differentiation.


Conditioned Medium Thyroid Cell Produce Growth Factor Radioreceptor Assay Iodide Uptake 
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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • William W. Mak
    • 1
  • Banani Bhaumick
    • 3
  • R. Marvin Bala
    • 3
  • Jeffrey E. Kudlow
    • 2
  • Gerard N. Burrow
    • 1
  1. 1.Banting and Best Department of Medical ResearchUniversity of TorontoSaskatoonCanada
  2. 2.Department of Clinical BiochemistryUniversity of TorontoSaskatoonCanada
  3. 3.Department of MedicineUniversity of SaskatchewanSaskatoonCanada

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