Production of Both Types of Insulin-Like Growth Factors by Primary Cultures of Ovine Thyroid Cells
One of the major tenets of thyroidology concerns TSH control of thyroid gland growth (1). The suppression of goiter by thyroid hormone is based on this premise. However, the effect of TSH on growth has been variable in different thyroid cell cultures in vitro. Recent advances in cell culture technique have allowed the culturing of ovine thyroid cells in chemically-defined, serum-free medium and, thus, the study on the role of TSH on thyroid growth and differentiation in the absence of serum factors. In primary cultures of ovine thyroid cells, TSH stimulates a number of differentiated aspects of thyroid function including follicle formation, iodide uptake and organification, cAMP production, thyroglobulin, thyroxine and triiodothyronine synthesis (2), as well as production of plasminogen activator (3). But, TSH does not stimulate growth in these ovine cells. The possibility existed that TSH did not stimulate growth directly. Conceivably, thyroid cells were producing growth factors as an aspect of differentiation.
KeywordsConditioned Medium Thyroid Cell Produce Growth Factor Radioreceptor Assay Iodide Uptake
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- 1.Dumont JE. In RS Harris, PL Manson, E Diczfalusy, et al. (eds), Vitamins and Hormones, Academic Press, New York, Vol. 29, 1971, p 287.Google Scholar
- 2.Eggo MC, Mak WW, Bachrach LK, et al. In MC Eggo and GN Burrow (eds), Thyroglobulin - The Prothyroid Hormone, Raven Press, New York, 1985, p 201.Google Scholar
- 7.Bala RM, Bhaumick B, Armstrong GH, et al. In EM Spencer (ed), Insulin-like Growth Factors Somatomedins, Walter de Gruyter, Berlin, 1983, p 491.Google Scholar
- 9.Yang YW-H, Acquaviva AM, Bruni CB, et al. In EM Spencer (ed), Insulin-like Growth Factors Somatomedins, Walter de Gruyter, Berlin, 1983, p 603.Google Scholar
- 10.Nissley SP and Rechler MM. Horm Prot Peptides 12: 127, 1985.Google Scholar