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Immunoreactive TRH and TRH-OH in Rat Hypophysial Portal Blood

  • Viviane Guillaume
  • L’Houcine Ouakif
  • Anne Dutour
  • Elias Castanas
  • Charles Oliver

Abstract

The release of the hypothalamic thyrotropin-releasing hormone (TRH) can be studied in the rat directly by radioimmunoassay in blood collected from the hypophysial portal vessels. Since TRH is rapidly inactivated in blood, it is necessary to collect hypophysial portal blood (HPB) in a way that minimizes TRH inactivation. In the present study, we have tested the validity of two different methods of collection of HPB for studying TRH secretion. Thereafter, we have measured TRH and its putative metabolite TRH-OH (Pyroglu-His-Pro-OH or Deamido-TRH) in HPB under various physiological and pharmacological conditions.

Keywords

Systemic Blood Pituitary Stalk Roid Hormone Methanol Solu Pharmacological Condition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Viviane Guillaume
    • 1
  • L’Houcine Ouakif
    • 1
  • Anne Dutour
    • 1
  • Elias Castanas
    • 1
  • Charles Oliver
    • 1
  1. 1.Laboratoire de Neuroendocrinologie ExperimentaleCNRS UA 560, Faculte de Medecine NordMarseilleFrance

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