Immunoreactive TRH and TRH-OH in Rat Hypophysial Portal Blood
The release of the hypothalamic thyrotropin-releasing hormone (TRH) can be studied in the rat directly by radioimmunoassay in blood collected from the hypophysial portal vessels. Since TRH is rapidly inactivated in blood, it is necessary to collect hypophysial portal blood (HPB) in a way that minimizes TRH inactivation. In the present study, we have tested the validity of two different methods of collection of HPB for studying TRH secretion. Thereafter, we have measured TRH and its putative metabolite TRH-OH (Pyroglu-His-Pro-OH or Deamido-TRH) in HPB under various physiological and pharmacological conditions.
KeywordsSystemic Blood Pituitary Stalk Roid Hormone Methanol Solu Pharmacological Condition
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- 6.Dutor A, Ouakif LH, Castanas E, et al. Life Sciences, 1985 (in press).Google Scholar