Cholinergic Agents and Inhibitors of Extracellular Calcium Influx Suppress the Secretion of TSH by Human Thyrotropic Adenoma Cells Maintained in Longterm Culture

  • Henry G. Fein
  • Nancy E. Butkus
  • Robert C. Smallridge


Recent reports from various centers have described effects of muscarinic agonists and blockers on anterior pituitary secretion in both animals and humans (1). Studies of intact organisms are limited, however, by the frequent significant stress such agents induce (2). Although important effects have been demonstrated for other pituitary hormones (3,4), reports of cholinergic effects on TSH secretion are scant (1). Thus, our laboratory has conducted ongoing studies of cholinergic effects on human TSH secretion in vitro in monolayer culture of cells obtained from TSH-secreting pituitary adenomas (5). In this paper, we report that incubation with acetylcholine inhibited basal and TRH-stimulated TSH secretion; only the latter effects were Blocked by addition of atropine.


Pituitary Adenoma Monolayer Culture Basal Secretion Cholinergic Agent Cholinergic Effect 
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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Henry G. Fein
    • 1
  • Nancy E. Butkus
    • 1
  • Robert C. Smallridge
    • 1
  1. 1.Division of MedicineWalter Reed Army Institute of ResearchUSA

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