Advertisement

Studies of Calmodulin-Like Portion in the TSH Receptor

  • Takashi Hachiya
  • Yoshiyuki Nakajima
  • Yasuaki Ura
  • Masao Ishida
  • Tadayoshi Miyazaki
  • Yukio Ochi

Summary

Calmodulin (CaM) antagonists, such as W–7, W–5 chlorpromazine, and haloperidol, inhibited dose-dependently 125I–bTSH binding to its receptor. This inhibitory effect by CaM antagonist was diminished by the addition of EDTA. Not only anti–CaM antibody but also CaM inhibited dose–dependently 1251–bTSH binding to its receptor. These results may indicate the presence of a CaM–like structure in the membrane receptor for TSH.

Four sera out of 300 patients with Graves’ disease had increased CaM binding activities as compared to normal control subjects. The binding could be demonstrated as the autoantibody to CaM by double antibody method, polyethyleneglycol method, and also gel–filtration. Three antibodies were monoclonal and one was polyclonal immunoglobulins (IgG and/or IgA).

Although the incidence of the autoantibody to CaM in Graves’ disease is low and the pathologiccal significance of the autoantibody to CaM remains obscure, the existence of this antibody in the serum of patients may suggest the production of antibody to a CaM–like structure in the TSH receptor.

Keywords

Subacute Thyroiditis Thyroid Receptor Polyclonal Immunoglobulin Thyroid Plasma Membrane Light Chain Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Hídaka H, Yamaki T, Naka M, et al. Mol Pharmacol 17: 66, 1980.PubMedGoogle Scholar
  2. 2.
    Rees Smith B and Hall R. Methods Enzymol 74: Pt. C, 405, 1981.CrossRefGoogle Scholar
  3. 3.
    Kajita Y, Nakajima Y, Ishida M, et al. Endocrinol Japon 31: 369, 1984.CrossRefGoogle Scholar
  4. 4.
    Kajita Y, Nakajima Y, Ishida M, et al. Acta Endocrinol 104: 423, 1983PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Takashi Hachiya
    • 1
  • Yoshiyuki Nakajima
    • 3
  • Yasuaki Ura
    • 3
  • Masao Ishida
    • 3
  • Tadayoshi Miyazaki
    • 2
  • Yukio Ochi
    • 4
  1. 1.Internal MedicineKyoto Pref. Univ. of MedicineJapan
  2. 2.RadiologyKyoto Pref. Univ. of MedicineJapan
  3. 3.Central Clin. Lab.Nantan General HospitalJapan
  4. 4.Shiga Univ. of Medical ScienceJapan

Personalised recommendations