A Highly Sensitive, Non-Isotopic Assay for Human TSH with Extended Clinical Utility
Serum thyrotropin (TSH) is widely regarded as the best indicator of thyroid function. The ideal clinical TSH assay would combine features of a short incubation time with precise measurement over a range encompassing hyperthyroid and hypothyroid values. Currently, most commercial assays have sensitivity limitations and, therefore, are relegated solely to the confirmation of primary hypothyroidism (1). The ultrasensitive research assays have displayed excellent delineation between euthyroid and hyperthyroid individuals but also possess time and range limitations (2–4). We report the development of a sensitive and precise immunoenzymometric assay (IEMA or EIA) for TSH with an extended clinical range. In a controlled clinical study, the EIA was compared to an ultrasensitive TSH RIA (UST) developed by Spencer and Nicoloff (2).
KeywordsPrimary Hypothyroidism Short Incubation Time Serum Thyrotropin Thyroid Hormone Replacement Therapy Southern California School
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