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A Highly Sensitive, Non-Isotopic Assay for Human TSH with Extended Clinical Utility

  • Robert C. Doss
  • B. J. Green
  • Carole A. Spencer
  • John T. Nicoloff

Abstract

Serum thyrotropin (TSH) is widely regarded as the best indicator of thyroid function. The ideal clinical TSH assay would combine features of a short incubation time with precise measurement over a range encompassing hyperthyroid and hypothyroid values. Currently, most commercial assays have sensitivity limitations and, therefore, are relegated solely to the confirmation of primary hypothyroidism (1). The ultrasensitive research assays have displayed excellent delineation between euthyroid and hyperthyroid individuals but also possess time and range limitations (2–4). We report the development of a sensitive and precise immunoenzymometric assay (IEMA or EIA) for TSH with an extended clinical range. In a controlled clinical study, the EIA was compared to an ultrasensitive TSH RIA (UST) developed by Spencer and Nicoloff (2).

Keywords

Primary Hypothyroidism Short Incubation Time Serum Thyrotropin Thyroid Hormone Replacement Therapy Southern California School 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Burger HG and Patel YC. Med J Australia 2: 293, 1972.PubMedGoogle Scholar
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    Spencer CA and Nicoloff JT. Clin Chim Acta 108: 415, 1980.PubMedCrossRefGoogle Scholar
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    Wehmann RE, Rubenstein HA, Pugeat MM, et al. Southern Med J 76: 969, 1983.PubMedCrossRefGoogle Scholar
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    Pekary AE, Hershman TM, and Parlow AF. J Clin Endocrinol Metab 41: 676, 1975.PubMedCrossRefGoogle Scholar
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    Rodbard D. Anal Biochem 90: 1, 1978.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Robert C. Doss
    • 2
  • B. J. Green
    • 2
  • Carole A. Spencer
    • 1
  • John T. Nicoloff
    • 1
  1. 1.The Clinical Research Unit, Department of MedicineThe University of Southern California School of MedicineLos AngelesUSA
  2. 2.Abbott LaboratoriesNorth ChicagoUSA

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