Characterization of the Optimal Thyrotropin (TSH) Induced Cyclic AMP Accumulation in the Cloned Human Thyroid Cell Line (GEJ)

  • Jean Jacques Remy
  • Jean Salamero
  • Jeanine Charreire


Cloned human thyroid epithelial cells, which we previously selected for their capacity to bind 125I-TSH to their TSH receptors, can produce cAMP in a dose-dependent manner, when stimulated by either TSH or GD IgG.

This cell line provides an effective tool which is both easily available and highly efficient and which, in addition, circumvents the need for human thyroid tissue, thyroid membranes, or receptor preparations. Moreover, this material excludes the possibility that species specificities reduce the chances of measuring a positive response.


Thyroid Cell cAMP Production Human Thyroid Human Thyroid Tissue Thyroid Cell Line 


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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Jean Jacques Remy
    • 1
  • Jean Salamero
    • 1
  • Jeanine Charreire
    • 1
  1. 1.INSERM U 283Hopital CochinParisFrance

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