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3,3′,5′-Triiodothyronine (rT3) Inhibits Iodothyronine-5′-Deiodinating Activity Induced by Equimolar Concentration of 3,5,3′-Triiodothyronine (T3) in Cultured Fetal Liver of Mouse

  • Doo Chol Han
  • Kanji Sato
  • Yuko Fujii
  • Toshio Tsushima
  • Kazou Shizume

Abstract

rT3 is generally considered to be without thyroid hormone activity at physiological concentrations and to play no significant physiological role. However, several agonistic and antagonistic effects of rT3 were reported at supraphysiological doses (1,2). As is well known, there exists little or no iodothyronine-5′-deiodinating activity (I-5′-DA) in the fetal liver of rodents (3). The enzyme activity rapidly increases after birth and peaks at 3–4 weeks in the case of mouse (4). We recently demonstrated that this ontogenetic development of iodothyronine-5′-deiodinase in the liver can also be induced in vitro by physiological concentrations of glucocorticoid and thyroid hormones (5). To investigate the rT3 effect on I-5′-DA, fetal liver of mouse on the 19th day of gestation, in which little or no I-5′-DA was detected and, therefore, rT3 was very stable, was cultured for 3–15 days, and I-5′-DA was determined in liver homogenate.

Keywords

Thyroid Hormone Fetal Liver Liver Homogenate Thyroid Hormone Activity Supraphysiological Dose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1986

Authors and Affiliations

  • Doo Chol Han
    • 1
  • Kanji Sato
    • 1
  • Yuko Fujii
    • 1
  • Toshio Tsushima
  • Kazou Shizume
    • 1
  1. 1.Institute of Clinical EndocrinologyTokyo Women’s Medical CollegeShinjuku, TokyoJapan

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