Inhibitory Effect of Thyroid Hormone on Protein Synthesis by Fetal Rat Neurons in Primary Culture
The effect of thyroid hormones on protein synthesis by fetal cortical cells in primary culture was studied by assessing (3H)-phenylalanine incorporation into trichloroacetic acid precipitable proteins. 48 h incubation with thyroid hormones, concentrations between 10−12 M and 10−7 M, produced a dose-dependent inhibition of protein synthesis. The time course study showed that T4 effect needed a longer period than T3 to achieve a significant inhibition of labeled amino acid incorporation into proteins. 48 h incubation of the cells with T3 (10−7 M) plus Actinomycin-D (5 μg/ml) resulted in a significant potentiation of T3 inhibitory effect. These results indicate that thyroid hormones inhibit total protein synthesis in fetal rat neurons.
KeywordsThyroid Hormone Total Protein Synthesis Significant Potentiation Tadpole Tail Assoc Manual
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