Initiation of Chloroplast DNA Replication in Chlamydomonas Reinhardtii

  • Madeline Wu
  • J. K. Lou
  • C. H. Chang
  • Z. Q. Nie
  • X. M. Wang
Part of the Basic Life Sciences book series (BLSC, volume 40)


The chloroplast is a self-duplicating extrachromosomal organelle with its own hereditary apparatus. Chloroplast DNAs from higher plants and green algae have been isolated as covalently closed circular molecules with lengths in the range of 110 to 215 kb. Chloroplasts contain multiple copies of the circular genome (1). Chloroplast DNA can be purified on the basis of either its location within the organelle or its distinct base composition. Nuclear and chloroplast genomes differ in the degree of control exerted over their replication, recombination, and segregation (2,8,20).


Chloroplast Genome Replication Origin Euglena Gracilis Prokaryotic Promoter Replication Loop 


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  1. 1.
    Bedbrook, J.R., and R.D. Kolodner (1979) The structure of chloroplast DNA. Am. Rev. Plant Physiol. 30:593–620.CrossRefGoogle Scholar
  2. 2.
    Birky, Jr., C.W. (1984) Relaxed cellular controls and organelle heredity. Science 222:468–475.Google Scholar
  3. 3.
    Chiang, K.S., and N. Sueoka (1967) Replication of chloroplast DNA in Chlamydomonas reinhardtii during the vegetative cell cycle: Its mode and regulation. Proc. Natl. Acad. Sci., USA 57:1506–1513.PubMedCrossRefGoogle Scholar
  4. 4.
    Chua, N.H., G. Blobel, P. Siekevitz, and G.E. Palade (1973) Attachment of chloroplast polysomes to thylakoid membranes in Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci., USA 70:1554–1558.PubMedCrossRefGoogle Scholar
  5. 5.
    Conrad, S.E., M. Wold, and J.L. Campbell (1979) Origin and direction of DNA replication of plasmid RSF 1030. Proc. Natl. Acad. Sci., USA 76:736–740.PubMedCrossRefGoogle Scholar
  6. 6.
    Edwards, K., and H. Kossel (1981) The rRNA operon from Zea mays chloroplasts: Nucleotide sequence of 23S rDNA and its homology with E. coli 23S rDNA. Nucl. Acids Res. 9:2853–2867.PubMedCrossRefGoogle Scholar
  7. 7.
    Fuller, R.S., J.M. Kaguni, and A. Romberg (1981) Enzymatic replication of the origin of the Escherichia coli chromosome. Proc. Natl. Acad. Sci., USA 78:7370–7374.PubMedCrossRefGoogle Scholar
  8. 8.
    Gillham, N.W. (1978) Organelle Heredity, Raven Press, New York.Google Scholar
  9. 9.
    Koller, B., and H. Delius (1982) Origin of replication in chloroplast DNA of Euglena gracilis located close to the region of variable size. EMBO J. 1:995–998.PubMedGoogle Scholar
  10. 10.
    Kolodner, R.D., and K.K. Tewari (1975) Chloroplast DNA from higher plants replicates by both the Cairns and rolling circle mechanism. Nature (London) 256:708–711.CrossRefGoogle Scholar
  11. 11.
    Kolodner, R.D., and K.K. Tewari (1975) Presence of displacement loops in the covalently closed circular chloroplast DNA from higher plants. J. Biol. Chem. 250:4888–4895.PubMedGoogle Scholar
  12. 12.
    Loppes, R., and C. Denis (1983) Chloroplast and nuclear fragments from Chlamydomonas promoting high frequency transformation of yeast. Current Genetics 7:473–480.CrossRefGoogle Scholar
  13. 13.
    Lothers, H., R. Rolling, C. Kucherer, and M. Schauzu (1985) A protein-regulated transcription: Effects on the in vitro replication of Escherichia coli minichromosomes. EMBO J. 4:555–560.Google Scholar
  14. 14.
    Maxam, A.M., and H. Gilbert (1977) A new method for sequencing DNA. Proc. Natl. Acad. Sci., USA 74:560–564.PubMedCrossRefGoogle Scholar
  15. 15.
    Orozco, Jr., E.M., J.E. Mullet, and N.H. Chua (1985) An in vitro system for accurate transcription initiation of chloroplast protein genes. Nucl. Acids Res. 13:1283–1302.PubMedCrossRefGoogle Scholar
  16. 16.
    Ravel-Chapuis, P., P. Heizman, and V. Nigon (1982) Electron microscopic localization of the replication origin of Euglena gracilis chloroplast DNA. Nature 300:78–81.CrossRefGoogle Scholar
  17. 17.
    Rochaix, J.-D. (1978) Restriction endonuclease map of the chloroplast DNA of Chlamydomonas reinhardtii. J. Mol. Biol. 126:567–617.CrossRefGoogle Scholar
  18. 18.
    Rochaix, J.-D., J. van Dillewijn, and M. Rahire (1984) Construction and characterization of autonomously replicating plasmids in the green unicellular alga Chlamydomonas reinhardtii. Cell 36:925–931.PubMedCrossRefGoogle Scholar
  19. 19.
    Rosenberg, M., A.B. Chepelinsky, and K. McKenney (1983) Studying promoters and terminators by gene fusion. Science 222:734–739.PubMedCrossRefGoogle Scholar
  20. 20.
    Sager, R. (1972) Cytoplasmic Genes and Organelles, Academic Press, New York.Google Scholar
  21. 21.
    Sager, R., and M.A. Ishida (1963) Chloroplast DNA in Chlamydomonas. Proc. Natl. Acad. Sci., USA 50:725.PubMedCrossRefGoogle Scholar
  22. 22.
    Sanger, F., S. Nicklen, and A. Coulson (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci., USA 74:5463–5467.PubMedCrossRefGoogle Scholar
  23. 23.
    Schlunegger, B., and E. Stutz (1984) The Euglena gracilis chloroplast genome: Structural features of a DNA region possibly carrying the single origin of DNA replication. Current Genetics 8:629–634.CrossRefGoogle Scholar
  24. 24.
    Seiki, M., N. Ogasawara, and H. Yoshikawa (1982) Identification of a suppressor sequence for DNA replication in the replication origin of the Bacillus subtilis chromosome. Proc. Natl. Acad. Sci., USA 79: 4285–4289.PubMedCrossRefGoogle Scholar
  25. 25.
    Tanaka, M., and S. Hiraga (1985) Negative control of oriC plasmid replication by transcription of the oriC region. Mol. Gen. Genet. 200: 21–26.PubMedCrossRefGoogle Scholar
  26. 26.
    Tinoco, Jr., I., P.N. Borer, B. Dangler, M.D. Levine, O.C. Uhlenbeck, D.M. Crothers, and J. Gralla (1973) Improved estimation of secondary structure in ribonucleic acids. Nature New Biology 246:40–41.PubMedGoogle Scholar
  27. 27.
    Vallet, J.-M., and J.-D. Rochaix (1985) Chloroplast origins of DNA replication are distinct from chloroplast ARS sequences in two green algae. Current Genetics 9:321–324.CrossRefGoogle Scholar
  28. 28.
    Vallet, J.-M., M. Rahire, and J.-D. Rochaix (1984) Localization and sequence analysis of chloroplast DNA sequences of Chlamydomonas reinhardtii that promote autonomous replication in yeast. EMBO J. 3:415–421.PubMedGoogle Scholar
  29. 29.
    Waddell, J., W. Wang, and M. Wu (1984) Electron microscopic localization of the chloroplast DNA replicative origins in Chlamydomonas reinhardtii. Nucl. Acids Res. 12:3843–3856.PubMedCrossRefGoogle Scholar
  30. 30.
    Wang, W., C.H. Chang, J. Waddell, and M. Wu (1984) Cloning and delimiting one chloroplast DNA replicative origin of Chlamydomonas. Nucl. Acids Res. 12:3857–3872.PubMedCrossRefGoogle Scholar
  31. 31.
    Watson, J.C., and S.J. Surzycki (1982) Extensive sequence homology in the DNA coding for elongation factor Tu from E. coli and C. reinhardtii chloroplast. Proc. Natl. Acad. Sci., USA 79:2264–2267.PubMedCrossRefGoogle Scholar
  32. 32.
    Wu, M., X.F. Kong, and S.D. Rung (1986) Prokaryotic promoters in the chloroplast DNA replication origin of Chlamydomonas reinhardtii. Current Genetics (in press).Google Scholar
  33. 33.
    Wu, M., J.K. Lou, D.Y. Chang, C.H. Chang, and Z.Q. Nie (1986) Structure and function of a chloroplast DNA replication origin of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci., USA (submitted for publication).Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Madeline Wu
    • 1
  • J. K. Lou
    • 1
  • C. H. Chang
    • 1
  • Z. Q. Nie
    • 1
  • X. M. Wang
    • 1
  1. 1.Department of Biological SciencesUniversity of Maryland Baltimore CountyCatonsvilleUSA

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