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The Mitochondrial DNA of Neurospora Crassa: Deletion by Intramolecular Recombination and the Expression of Mitochondrial Genes

  • Hans de Vries
  • Cobi Schrage
  • Jenny C. De Jonge
Part of the Basic Life Sciences book series (BLSC, volume 40)

Abstract

Stopper (stp) mutants of Neurospora crassa are characterized by an irregular stop-start growth pattern on agar plates or in tubes (3). All stoppers isolated so far have grossly abnormal mitochondrial DNA (mtDNA) (3,9,13) and are deficient in respiratory chain components. The mutant stp[E35], isolated in our laboratory, is an example of this category (9, 10). Cytochromes aa 3 and b are virtually absent in this mutant, and cytochrome c oxidase activity is only marginal (about 3% of wild-type activity). We have shown (10) that the absence of cytochrome c oxidase is not due to a blocked synthesis of its subunits, but rather to a defect in their assembly.

Keywords

Mitochondrial Genome tRNA Gene Neurospora Crassa Electron Paramagnetic Reso Stopper Mutant 
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References

  1. 1.
    Anderson, S., A. T. Bankier, B.G. Barrell, M.H.L. de Bruijn, A.R. Coulson, J. Drouin, I.C. Eperon, D.P. Nierlich, B.A. Roe, F. Sanger, P.H. Schreier, A.J.N. Smith, R. Staden, and I.G. Young (1981) Sequence and organization of the human mitochondrial genome. Nature 290:457–465.PubMedCrossRefGoogle Scholar
  2. 2.
    Bernardi, G. (1982) The origins of replication of the mitochondrial genome of yeast. Trends Biochem. Sci. 7:404–408.CrossRefGoogle Scholar
  3. 3.
    Bertrand, H., R.A. Collins, L.L. Stohl, R.R. Goewert, and A.M. Lambowitz (1980) Deletion mutants of Neurospora crassa mitochondrial DNA and their relation to the “stop-start” growth phenotype. Proc. Natl. Acad. Sci., USA 77:6032–6036.PubMedCrossRefGoogle Scholar
  4. 4.
    Burger, G., M. Helmer-Citterich, M.A. Nelson, S. Werner, and G. Macino (1985) RNA processing in Neurospora crassa mitochondria: Transfer RNAs punctuate a large precursor transcript. EMBO J. 4:197–204.PubMedGoogle Scholar
  5. 5.
    Cech, T.R., N.K. Tanner, L. Tinoco, B.R. Wier, M. Zuker, and P.S. Perlman (1983) Secondary structure of the Tetrahymena ribosomal RNA intervening sequence: Structural homology with fungal mitochondria intervening sequences. Proc. Natl. Acad. Sei., USA 80:3903–3907.CrossRefGoogle Scholar
  6. 6.
    Chomyn, A., P. Mariottini, M.W.J. Cleeter, C.I. Ragan, A. Matsuno-Yagi, Y. Hatefi, R.F. Doolittle, and G. Attardi (1985) Six unidentified reading frames of human mitochondrial DNA encode components of the respiratory-chain NADH dehydrogenase. Nature 314:592–597.PubMedCrossRefGoogle Scholar
  7. 7.
    Davies, R.W., R.B. Waring, J.A. Ray, T.A. Brown, and C. Scazzochio (1982) Making ends meet: A model for RNA splicing in fungal mitochondria. Nature 300:719–724.PubMedCrossRefGoogle Scholar
  8. 8.
    de Vries, H., J.C. De Jonge, and C. Schrage (1985) The Neurospora mitochondrial stopper mutant, E35, lacks two protein genes indispensable for the formation of complexes I, III, and IV. In Achievements and Perspectives of Mitochondrial Research. Vol. II. Biogenesis, E. Quagliariello, E.C. Slater, F. Palmieri, C. Saccone, and A.M. Kroon, eds. Elsevier, Amsterdam, pp. 285–292.Google Scholar
  9. 9.
    de Vries, H., J.C. De Jonge, and P. van’t Sant (1980) Characterization of a mitochondrial “stopper” mutant of Neurospora crassa; Deletions and rearrangements in the mitochondrial DNA result in disturbed assembly of respiratory chain components. In The Organization and Expression of the Mitochondrial Genome, A.M. Kroon and C. Saccone, eds. Elsevier/North-Holland, Amsterdam, pp. 333–342.Google Scholar
  10. 10.
    de Vries, H., J.C. De Jonge, P. van’t Sant, E. Agsteribbe, and A. Arnberg (1981) A “stopper” mutant of Neurospora crassa containing two populations of aberrant mitochondrial DNA. Curr. Genet. 3:205–211.CrossRefGoogle Scholar
  11. 11.
    de Vries, H., B. Alzner-de Weerd, CA. Breitenberger, D.D. Chang, J.C. De Jonge, and U.L. RajBhandary (1986) The E35 stopper mutant of Neurospora crassa: Precise localization of deletion endpoints in mitochondrial DNA and evidence that the deleted DNA codes for a subunit of NADH dehydrogenase. EMBO J. 5:779–785.PubMedGoogle Scholar
  12. 12.
    de Zamaroczy, M., G. Faugeron-Fonty, and G. Bernardi (1983) Excision sequences in the mitochondrial genome of yeast. Gene 21:193–202.PubMedCrossRefGoogle Scholar
  13. 13.
    Gross, S.R., T.-S. Hsieh, and P.H. Levine (1984) Intramolecular recombination as a source of mitochondrial chromosome heteromorphism in Neurospora. Cell 38:233–239.PubMedCrossRefGoogle Scholar
  14. 14.
    Hensgens, L.A.M., L. Bonen, M. de Haan, G. van der Horst, and L.A. Grivell (1983) Two intron sequences in yeast mitochondrial CO I gene: Homology among URF-containing introns and strain-dependent variation in flanking exons. Cell 32:379–389.PubMedCrossRefGoogle Scholar
  15. 15.
    Ise, W., H. Haiker, and H. Weiss (1985) Mitochondrial translation of subunits of the rotenone-sensitive NADH-ubiquinone reduction in Neurospora crassa. EMBO J. 4:2075–2080.PubMedGoogle Scholar
  16. 16.
    Ojala, D., J. Montoya, and G. Attardi (1981) tRNA punctuation model of RNA processing in human mitochondria. Nature 290:470–474.PubMedCrossRefGoogle Scholar
  17. 17.
    Van den Bogert, C., M. Holtrop, H. de. Vries, and A.M. Kroon (1985) Specific inhibition of mitochondrial protein synthesis influences the amount of complex I in mitochondria of rat liver and Neurospora crassa directly. FEBS Lett. 192:225–229.PubMedCrossRefGoogle Scholar
  18. 18.
    Van den Bogert, C., M. Holtrop, T. Melis, H. de Vries, S.P.J. Albracht, and A.M. Kroon (1985) The effect of long-term inhibition of mitochondrial protein synthesis on the oxidation capacity of mitochondria for NADH-linked substrates. In Achievements and Perspectives of Mitochondrial Research. Vol. II. Biogenesis, E. Quagliariello, E.C. Slater, F. Palmieri, C. Saccone, and A.M. Kroon, eds. Elsevier, Amsterdam, pp. 277–284.Google Scholar
  19. 19.
    Van den Boogaart, P., J. Samallo, and E. Agsteribbe (1982) Similar genes for a mitochondrial ATPase subunit in the nuclear and mitochondrial genomes of Neurospora crassa. Nature 298:187–189.PubMedCrossRefGoogle Scholar
  20. 20.
    Yin, S., J. Heckman, and U.L. RajBhandary (1981) Highly conserved GC-rich palindromic DNA sequences flank tRNA genes in Neurospora crassa. Cell 26:325–332.PubMedCrossRefGoogle Scholar
  21. 21.
    Yin, S., J. Burke, D.D. Chang, K.S. Browning, J.E. Heckman, B. Alznerde Weerd, M.J. Potter, and U.L. RajBhandary (1982) Neurospora crassa mitochondrial tRNAs and rRNAs: Structure, gene organization, and DNA sequences. In Mitochondrial Genes, P. Slonimski, P. Borst, and G. Attardi, eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp. 361–373.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Hans de Vries
    • 1
  • Cobi Schrage
    • 1
  • Jenny C. De Jonge
    • 1
  1. 1.Laboratory of Physiological ChemistryState University at GroningenGroningenThe Netherlands

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