The Mitochondrial DNA of Neurospora Crassa: Deletion by Intramolecular Recombination and the Expression of Mitochondrial Genes
Stopper (stp) mutants of Neurospora crassa are characterized by an irregular stop-start growth pattern on agar plates or in tubes (3). All stoppers isolated so far have grossly abnormal mitochondrial DNA (mtDNA) (3,9,13) and are deficient in respiratory chain components. The mutant stp[E35], isolated in our laboratory, is an example of this category (9, 10). Cytochromes aa 3 and b are virtually absent in this mutant, and cytochrome c oxidase activity is only marginal (about 3% of wild-type activity). We have shown (10) that the absence of cytochrome c oxidase is not due to a blocked synthesis of its subunits, but rather to a defect in their assembly.
KeywordsMitochondrial Genome tRNA Gene Neurospora Crassa Electron Paramagnetic Reso Stopper Mutant
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