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Gene Cloning and Mutant Isolation of Subunits of RNA Polymerases in the Yeast Saccharomyces Cerevisiae

  • Pierre Thuriaux
  • Carl Mann
  • Jean-Marie Buhler
  • Isabelle Treich
  • Rosmarie Gudenus
  • Sylvie Mariotte
  • Michel Riva
  • André Sentenac
Part of the Basic Life Sciences book series (BLSC, volume 40)

Abstract

Compared to the bacterial situation, the transcriptional machinery of eukaryotes is remarkably complex. This complexity is evident at three levels. First, there is a multiplicity of RNA polymerases (one mitochondrial enzyme and three nuclear enzymes, the latter being designated as RNA polymerases A, B, and C or I, II, and III),* whereas there is only one enzyme in all bacteria and archebacteria thus far analyzed. Each RNA polymerase has a distinct gene specificity, with RNA polymerase A (which is nucleolar) synthesizing the large ribosomal RNA, polymerase B (which is the most sensitive to a amanitin) producing messenger RNAs, and polymerase C synthesizing small RNAs, such as 5S RNA and transfer RNAs. Second, each nuclear RNA polymerase appears to have more different polypeptide subunits than the bacterial enzyme. Third, purified eukaryotic RNA polymerases are unable to carry the accurate transcription of isolated genes in vitro, which is in contrast to bacterial holoenzymes. There appears to be no equivalent to a subunits in eukaryotic RNA polymerases, where additional proteins (transcription factors) are required to reconstitute specific transcription.

Keywords

Null Allele Mutant Allele Replicative Plasmid Haploid Strain Mutant Isolation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Pierre Thuriaux
    • 1
  • Carl Mann
    • 1
  • Jean-Marie Buhler
    • 1
  • Isabelle Treich
    • 1
  • Rosmarie Gudenus
    • 1
  • Sylvie Mariotte
    • 1
  • Michel Riva
    • 1
  • André Sentenac
    • 1
  1. 1.Service de BiochimieCentre d’Etudes Nucléaires de SaclayGif-sur-YvetteFrance

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