Advertisement

Regulation of the Yeast CYC1 Gene

  • Leonard P. Guarente
Part of the Basic Life Sciences book series (BLSC, volume 40)

Abstract

Evidence is increasingly accumulating to indicate that eukaryotic RNA polymerases do not recognize promoter sequences encoded in DNA as does their prokaryotic counterpart. Rather, eukaryotic promoter/regulatory regions encompass sites that bind multiple transcription factors. Some of these factors are “mechanical” in nature, such as the TATA box factor required for genes transcribed by RNA polymerase II. Other factors are gene-specific and bind to sequences found upstream of the TATA box. The interplay of the latter class of factors with regulatory sequences is responsible for regulation of transcription.

Keywords

Initiation Site CYCI Gene TATA Element Yeast Promoter Nonfermentable Carbon Source 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Arcangioli, B., and B. Lescure (1985) Identification of proteins involved in the regulation of yeast iso-1-cytochrome c expression oxygen. EMBO J. 4:2627–2633.PubMedGoogle Scholar
  2. 2.
    Bram, R.J., and R.D. Romberg (1985) Specific protein binding to far upstream activating sequences in polymerase II promoters. Proc. Natl. Acad. Sci., USA 82:43–47.PubMedCrossRefGoogle Scholar
  3. 3.
    Faye, G., D. Leung, K. Tatchell, B. Hall, and B. Smith (1981) Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene. Proc. Natl. Acad. Sci., USA 78:2258–2262.PubMedCrossRefGoogle Scholar
  4. 4.
    Ginegar, E., S.M. Varnum, and M. Ptashne (1985) Specific DNA binding of GAL4, a positive regulatory protein of yeast. Cell 48:676–774.Google Scholar
  5. 5.
    Guarente, L. (1984) Yeast promoters: Positive and negative elements. Cell 36:799–800.PubMedCrossRefGoogle Scholar
  6. 6.
    Guarente, L., and E. Hoar (1984) Upstream activation sites of the CYC1 gene of Saccharomyces cerevisiae are active when inverted but not when placed downstream of the TATA box. Proc. Natl. Acad. Sci., USA 81: 7860–7864.PubMedCrossRefGoogle Scholar
  7. 7.
    Guarente, L., and T. Mason (1983) Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site. Cell 32:1279–1286.PubMedCrossRefGoogle Scholar
  8. 8.
    Guarente, L., B. Lalonde, P. Gifford, and E. Alani (1984) Distinctively regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S . cerevisiae. Cell 36:504–511.Google Scholar
  9. 9.
    Hahn, S., E. Hoar, and L. Guarente (1985) Each of three “TATA elements” specifies a subset of the transcription initiation sites at the CYC1 promoter of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci., USA 82:8562–8566.PubMedCrossRefGoogle Scholar
  10. 10.
    Hope, I., and K. Struhl (1985) GCN4 protein synthesized in vitro binds HIS3 regulatory sequences’ implications for general control of amino acid biosynthetic genes in yeast. Cell 43:177–188.PubMedCrossRefGoogle Scholar
  11. 11.
    Lalonde, B., B. Arcangioli, and L. Guarente (1986) A single yeast upstream activation site UAS1 has two distinct regions essential for its activity. Mol. Cell. Biol, (submitted for publication).Google Scholar
  12. 12.
    Pinkham, J.L., and L. Guarente (1985) Cloning and molecular analysis of the HAP2 locus: A global regulator of respiratory genes in S. cerevisiae. Mol. Cell. Biol. 5:3410–3416.PubMedGoogle Scholar
  13. 13.
    Sherman, F., and J. Stewart (1971) Genetics and biosynthesis of cytochromec. Ann. Rev. Genet. 5:257–296.PubMedCrossRefGoogle Scholar
  14. 14.
    Struhl, K. (1982) The yeast HIS3 promoter contains at least two distinct elements. Proc. Natl. Acad. Sci., USA 79:7385–7389.PubMedCrossRefGoogle Scholar
  15. 15.
    Struhl, K. (1984) Genetic properties and chromatin structure of the yeast gal regulatory element: An enhancer-like sequence. Proc. Natl. Acad. Sci., USA 81:7865–7869.PubMedCrossRefGoogle Scholar
  16. 16.
    Verdiere, J., F. Creusot, L. Guarente, and P.P. Slonimski (1986) The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromesc. Curr. Genet. 10:339–342.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Leonard P. Guarente
    • 1
  1. 1.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

Personalised recommendations