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Yeast DNA Replication

  • Chapter
Extrachromosomal Elements in Lower Eukaryotes

Part of the book series: Basic Life Sciences ((BLSC,volume 40))

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Abstract

We have explored various strategies for exploiting the yeast genetic and biochemical system for understanding DNA replication. Because of the long time that has intervened between the isolation of random replication mutants of yeast and the identification of the gene products affected, an alternative approach to elucidating the molecular basis of replication has been needed. One such alternative involves purifying replication proteins, isolating the genes encoding them, and constructing the appropriate mutant from the cloned gene. We have applied this “reverse genetics” strategy to three different replication activities: DNA polymerases, single-stranded DNA binding proteins, and proteins that bind to autonomously replicating sequence (ARS) elements.

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References

  1. Badaracco, G., L. Capucci, P. Plevani, and L.M.S. Chang (1983) Polypeptide structure of DNA polymerase I from Saccharomyces cerevisiae. J. Biol. Chem. 258:10720–10726.

    PubMed  CAS  Google Scholar 

  2. Beyer, A.J., M.E. Christensen, B.W. Walker, and W.M. LeStourgeon (1977) Identification and characterization of the packaging proteins of core 40S hnRNP particles thymidylate synthetase. Cell 11:127–138.

    Article  PubMed  CAS  Google Scholar 

  3. Bisson, I., and J. Thorner (1977) Thymidine 5’-monophosphate-requiring mutants of Saccharomyces cerevisiae are deficient in thymidylate synthetase. J. Bacteriol. 132:44–50.

    PubMed  CAS  Google Scholar 

  4. Bram, R.J., and R.D. Rornberg (1985) Specific protein binding for upstream activating sequences in polymerase II promoters. Proc. Natl. Acad. Sci., USA 82:43–47.

    Article  PubMed  CAS  Google Scholar 

  5. Campbell, J.L. (1983) Yeast DNA replication. In Genetic Engineering: Principles and Methods, Vol. 5, J.K. Setlow and A. Hollaender, eds. Plenum Press, New York, pp. 109–156.

    Google Scholar 

  6. Campbell, J.L. (1986) Eukaryotic DNA replication. Ann. Rev. Biochem. Vol. 55 (in press).

    Google Scholar 

  7. Celniker, S.E., K. Sweder, F. Srienc, J.E. Bailey, and J.L. Campbell (1984) Deletion mutations affecting autonomously replicating sequence ARS1 of Saccharomyces cerevisiae. Mol. Cell. Biol. 4:2455–2466.

    PubMed  CAS  Google Scholar 

  8. Chang, L.M.S. (1977) DNA polymerases from bakers’ yeast. J. Biol. Chem. 252:1873–1880.

    PubMed  CAS  Google Scholar 

  9. Chelm, B.D., and E.P. Geidushek (1979) Gel electrophoretic separation of transcription complexes: An assay for RNA polymerase selectivity and a method for promoting mapping. Nucl. Acids Res. 7:1851–1867.

    Article  PubMed  CAS  Google Scholar 

  10. Cobianchi, F., D.N. Sen Gupta, B.Z. Zmudzka, and S.H. Wilson (1986) Structure of rodent helix-destabilizing proteins revealed by cDNA cloning. J. Biol. Chem. 261:3536–3543.

    PubMed  CAS  Google Scholar 

  11. Dodson, M., J. Roberts, R. McMacken, and H. Echols (1985) Specialized nucleoprotein structures at the origin of replication of bacteriophage λ: Complexes with λO protein and with λO, λP, and Escherichia coli Dnaβ proteins. Proc. Natl. Acad. Sci., USA 82:4678–4682.

    Article  PubMed  CAS  Google Scholar 

  12. Dumas, L.B., J.P. Lussky, E.F. McFarland, and J. Shampay (1982) New temperature-sensitive mutants of Saccharomyces cerevisiae affecting DNA replication. Mol. Gen. Genet. 187:42–46.

    Article  PubMed  CAS  Google Scholar 

  13. Formosa, T., R. Burke, and B. Alberts (1983) Affinity purification of bacteriophage T4 proteins essential for DNA replication and genetic recombination. Proc. Natl. Acad. Sci., USA 80:2442.

    Article  PubMed  CAS  Google Scholar 

  14. Fried, M., and D. Crothers (1981) Equilibria and kinetics of Lac repressor-operator interactions by Polyacrylamide gel electrophoresis. Nucl. Acids Res. 9:6505–6525.

    Article  PubMed  CAS  Google Scholar 

  15. Fuller, R.S., B.E. Funnell, and A. Romberg (1984) The dnaA protein complex with the E. coli chromosomal replication origin (oriC) and other DNA sites. Cell 38:889–900.

    Article  PubMed  CAS  Google Scholar 

  16. Game, J.C. (1976) Yeast cell-cycle mutant cde21 is a temperature-sensitive thymidylate auxotroph. Mol. Gen. Genet. 146:313–315.

    Article  PubMed  CAS  Google Scholar 

  17. Garner, M.M, and A. Revzin (1981) A gel electrophoresis method for quantifying the bending of proteins to specific DNA regions: Application to components of the Escherichia coli lactose Operon regulating system. Nucl. Acids Res. 9:3047–3060.

    Article  PubMed  CAS  Google Scholar 

  18. Hartwell, L. (1974) Genetic control of the cell division cycle in yeast. J. Mol. Biol. 59:183–194.

    Article  Google Scholar 

  19. Herrick, G., and B. Alberts (1976) Purification and physical characterization of nucleic acid helix-unwinding proteins from calf thymus. J. Biol. Chem. 251:2124–2132.

    PubMed  CAS  Google Scholar 

  20. Herrick, G., and B. Alberts (1976) Nucleic acid helix-coil transition mediated by helix-unwinding proteins from calf thymus. J. Biol. Chem. 251:2133–2141.

    PubMed  CAS  Google Scholar 

  21. Herrick, G., and B. Alberts (1976) Single-stranded DNA structure and DNA polymerase activity in the presence of nucleic acid helix-unwinding proteins from calf thymus. J. Biol. Chem. 251:2142–2146.

    PubMed  CAS  Google Scholar 

  22. Hieter, P., C. Mann, M. Snyder, and R.W. Davis (1985) Mitotic stability of yeast chromosomes: A colony color assay that measures nondisjunction and chromosome loss. Cell 40:381–392.

    Article  PubMed  CAS  Google Scholar 

  23. Johnson, L.M., M. Snyder, L.M.S. Chang, R.W. Davis, and J.L. Campbell (1985) Isolation of the gene encoding yeast DNA polymerase I. Cell 43:369–377.

    Article  PubMed  CAS  Google Scholar 

  24. Johnston, L.H., and J.C. Game (1978) Mutants of yeast with depressed DNA synthesis. Mol. Gen. Genet. 161:205–214.

    PubMed  CAS  Google Scholar 

  25. Johnston, L.H., and K.A. Nasmyth (1978) Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase. Nature 274:891–894.

    Article  PubMed  CAS  Google Scholar 

  26. Jong, A.Y.S., and J.L. Campbell (1984) Characterization of Saccharomyces cerevisiae thymidylate kinase, the CDC8 gene product. J. Biol. Chem. 259:14394–14398.

    PubMed  CAS  Google Scholar 

  27. Jong, A.Y.S., and J.L. Campbell (1986) Isolation of the gene encoding yeast single-stranded nucleic acid binding protein I (SSB-1). Proc. Natl. Acad. Sci., USA 83:877–881.

    Article  PubMed  CAS  Google Scholar 

  28. Jong, A.Y.S., R. Aebersold, and J.L. Campbell (1985) Multiple species of single-stranded DNA binding proteins in Saccharomyces cerevisiae. J. Biol. Chem. 260:16367–16374.

    PubMed  CAS  Google Scholar 

  29. Jong, A.Y.S., C.L. Kuo, and J.L. Campbell (1984) The CDC8 gene of yeast encodes thymidylate kinase. J. Biol. Chem. 259:11052–11058.

    PubMed  CAS  Google Scholar 

  30. Kearsey, S. (1984) Structural requirements for the function of a yeast chromosomal replicator. Cell 37:299–307.

    Article  PubMed  CAS  Google Scholar 

  31. Koshland, D., J.C. Kent, and L.H. Hartwell (1985) Genetic analysis of the mitotic transmission of minichromosomes. Cell 40:393–403.

    Article  PubMed  CAS  Google Scholar 

  32. Kuo, C.L., and J.L. Campbell (1983) Isolation of yeast DNA replication mutants in permeabilized cells. Proc. Natl. Acad. Sci., USA 80:6465–6469.

    Article  PubMed  CAS  Google Scholar 

  33. LeStourgeon, W.M., A.L. Beyer, M.E. Christensen, B.W. Walker, S.M. Poupore et al. (1977) The packaging proteins of core hnRNP particles and the maintenance of proliferative cell states. Cold Spring Harbor Symp. Quant. Biol. 42:885–898.

    Article  Google Scholar 

  34. Merrill, B.M., M.B. LoPresti, K.L. Stone, and K.R. Williams (1986) High pressure liquid chromatography purification of UP1 and UP2, two related single-stranded nucleic acid-binding proteins from calf thymus. J. Biol. Chem. 261:878–883.

    PubMed  CAS  Google Scholar 

  35. Pandolfo, M., O. Valentini, G. Biamonti, C. Morandi, and S. Riva (1985) Single stranded DNA binding proteins derived from hnRNP proteins by proteolysis in mammalian cells. Nucl. Acids Res. 13:6577–6590.

    Article  PubMed  CAS  Google Scholar 

  36. Parker, C., and J. Topol (1984) A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA binding activity. Cell 36:357–369.

    Article  PubMed  CAS  Google Scholar 

  37. Plevani, P., G. Badaracco, C. Augh, and L.M.S. Chang (1984) DNA polymerase I and DNA primase complex in yeast. J. Biol. Chem. 259:7532–7539.

    PubMed  CAS  Google Scholar 

  38. Plevani, P., M. Foiari, P. Valsasnini, and G. Badaracco (1985) Polypeptide structure of DNA primase from a yeast DNA polymerase-primase complex. J. Biol. Chem. 260:7102–7107.

    PubMed  CAS  Google Scholar 

  39. Sapp, M., H. Konig, H.D. Riedl, A. Richter, and R. Knippers (1985) A newly detected class of mammalian single strand-specific DNA-binding proteins. J. Biol. Chem. 260:1550–1556.

    PubMed  CAS  Google Scholar 

  40. Sclafani, R.A., and W.L. Fangman (1984) Yeast gene CDC8 encodes thymidylate kinase and is complemented by herpes thymidine kinase gene TK. Proc. Natl. Acad. Sci., USA 81:5821–5825.

    Article  PubMed  CAS  Google Scholar 

  41. Shortle, D., P. Novick, and D. Dotstein (1984) Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene. Proc. Natl. Acad. Sci., USA 81:4889–4893.

    Article  PubMed  CAS  Google Scholar 

  42. Singh, H., and L.B. Dumas (1984) A DNA primase that copurifies with the major DNA polymerase from the yeast Saccharomyces cerevisiae. J. Biol. Chem. 259:7936–7940.

    PubMed  CAS  Google Scholar 

  43. Srienc, F., J.E. Bailey, and J.L. Campbell (1985) Effect of ARS1 mutations on chromosome stability in Saccharomyces cerevisiae. Mol. Cell. Biol. 5:1676–1684.

    PubMed  CAS  Google Scholar 

  44. Sugino, A., H. Kojo, B.D. Greenberg, P.O. Brown, and K.C. Kim (1981) In vitro replication of yeast 2-um plasmid DNA. In The Initiation ofDNA Replication (ICN-UCLA Symposia on Molecular and Cellular Biology, Vol. 11), Academic Press, Inc., New York, pp. 529–533.

    Google Scholar 

  45. Takanami, M., S. Tabata, A. Oka, K. Sugimoto, H. Sasaki, S. Yasuda, and Y. Hirota (1983) The Escherichia coli origin of replication: Essential structure for bidirectional replication. In Mechanisms of DNA Replication (ICN-UCLA Symposia on Molecular and Cellular Biology, Vol. 10), Alan R. Liss, Inc., New York, pp. 257–273.

    Google Scholar 

  46. Valentini, O., G. Biamonti, M. Pandolfo, C. Morandi, and S. Riva (1984) Mammalian single-stranded DNA binding proteins and heterogeneous nuclear RNA proteins have common antigenic determinants. Nucl. Acids Res. 13:337–346.

    Article  Google Scholar 

  47. Walker, J.M., K. Gooderham, J.B.B. Hastings, E. Mayes, and E.W. Johns (1980) The primary structures of non-histone chromosomal proteins HMG 1 and 2. FEBS Lett. 122:264–270.

    Article  PubMed  CAS  Google Scholar 

  48. Williams, K.R., E.K. Spicer, M.B. LoPresti, R.A. Guggenheimer, and J.W. Chase (1983) Limited proteolysis studies on the Escherichia coli single-stranded DNA binding protein. J. Biol. Chem. 258:3346–3355.

    PubMed  CAS  Google Scholar 

  49. Williams, K.R., K.L. Stone, M.B. LoPresti, B.M. Merrill, and S.R. Planck (1985) Amino acid sequence of the UP1 calf thymus helix-destabilizing protein and its homology to an analogous protein from mouse myeloma. Proc. Natl. Acad. Sci., USA 82:5666–5670.

    Article  PubMed  CAS  Google Scholar 

  50. Wintersberger, E. (1974) Absence of a low-molecular-weight DNA polymerase from nuclei of the yeast, Saccharomyces cerevisiae. Eur. J. Biochem. 50:197–202.

    Article  PubMed  CAS  Google Scholar 

  51. Wintersberger, E. (1978) Yeast DNA polmerases: Antigenic relationship, use of RNA primer and associated exonuclease activity. Eur. J. Biochem. 84:167–172.

    Article  PubMed  CAS  Google Scholar 

  52. Wintersberger, U., and E. Wintersberger (1970) Studies on deoxyribonucleic acid polymerases from yeast. 1. Partial purification and properties of two DNA polymerases from mitochondria-free cell extracts. Eur. J. Biochem. 13:11–19.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Divisions of Biology and Chemistry, California Institute of Technology, Pasadena, California, 91125, USA

    Judith L. Campbell, Martin Budd, Colin Gordon, Ambrose Jong, Kevin Sweder, Alexander Oehm & Mary Gilbert

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  1. Judith L. Campbell
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  2. Martin Budd
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  4. Ambrose Jong
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  5. Kevin Sweder
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  6. Alexander Oehm
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  7. Mary Gilbert
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Editor information

Editors and Affiliations

  1. National Institutes of Health, Bethesda, Maryland, USA

    Reed B. Wickner  & Alan Hinnebusch  & 

  2. Ohio State University, Columbus, Ohio, USA

    Alan M. Lambowitz

  3. University of Illinois, Urbana, Illinois, USA

    I. C. Gunsalus  & Richard I. Gumport  & 

  4. Conuncil for Reasearch Planning in Biological Sciences, Inc., Washington, D.C., USA

    Alexander Hollaender , Claire M. Wilson  & Gregory Kuny ,  & 

  5. Indiana University, Bloomington, Indiana, USA

    John R. Preer Jr.

  6. University of Chicago, Chicago, Illinois, USA

    Laurens Mets

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© 1986 Plenum Press, New York

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Campbell, J.L. et al. (1986). Yeast DNA Replication. In: Wickner, R.B., et al. Extrachromosomal Elements in Lower Eukaryotes. Basic Life Sciences, vol 40. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5251-8_35

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  • DOI: https://doi.org/10.1007/978-1-4684-5251-8_35

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4684-5253-2

  • Online ISBN: 978-1-4684-5251-8

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