Analysis and Utilization of the Preprotoxin Gene Encoded in the M1 Double-Stranded RNA of Yeast

  • Nigel Skipper
Part of the Basic Life Sciences book series (BLSC, volume 40)


The yeast type 1 toxin is a secreted protein which, along with immunity to the toxin, is encoded by an encapsidated double-stranded RNA (dsRNA) as a precursor protein, the preprotoxin. This chapter reviews recent work that has led to sequencing of complementary DNA (cDNA) copies of the preprotoxin gene, expression of toxin and toxin-Immunity from cDNA, and use of the cDNA to effect secretion from yeast of a bacterial cellulase.


Cellulase Gene BamHI Fragment Killer Yeast Killer Strain Killer Phenotype 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Nigel Skipper
    • 1
  1. 1.Division of Molecular BiologyAllelix, Inc.MississaugaCanada

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