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Sequence of the M1-2 Region of Killer Virus Double-Stranded RNA

  • Denise E. Georgopoulos
  • Ernest M. Hannig
  • Michael J. Leibowitz
Part of the Basic Life Sciences book series (BLSC, volume 40)

Abstract

A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.

Keywords

Negative Strand Positive Strand Internal Portion Yeast Killer dsRNA Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Denise E. Georgopoulos
    • 1
  • Ernest M. Hannig
    • 1
  • Michael J. Leibowitz
    • 1
  1. 1.Department of Microbiology, Rutgers Medical SchoolUniversity of Medicine and Dentistry of New JerseyPiscatawayUSA

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