Sequence of the M1-2 Region of Killer Virus Double-Stranded RNA

  • Denise E. Georgopoulos
  • Ernest M. Hannig
  • Michael J. Leibowitz
Part of the Basic Life Sciences book series (BLSC, volume 40)


A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.


Negative Strand Positive Strand Internal Portion Yeast Killer dsRNA Molecule 
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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Denise E. Georgopoulos
    • 1
  • Ernest M. Hannig
    • 1
  • Michael J. Leibowitz
    • 1
  1. 1.Department of Microbiology, Rutgers Medical SchoolUniversity of Medicine and Dentistry of New JerseyPiscatawayUSA

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