Abstract
We cloned MAK11, MAK18, and MKT1 utilizing their genetic map positions. The MAK11 gene is close to CDC16 on chromosome XI. Both genes were cloned on a single 7-kb fragment, and both have now been sequenced. The MAK18 gene is located close to PET3 on chromosome VIII. A large plasmid carrying PET3 was obtained from R. Elder and R.E. Esposito and was found to also have the MAK18 gene. The MAK16 gene has been subcloned and sequenced starting with a clone provided by J. Crowley and D. Kaback. The MKT1 gene was mapped near the gene for topoisomerase II. The topoisomerase II clone was used as the starting point for chromosome-walking to isolate MKT1. A deletion-insertion mutation (disruption) of MKT1 results in an inability to maintain M2, but does not affect M1 or L-A maintenance.
Clones of SKI3 and SKI8 were selected using the cold sensitivity for cell growth of ski ‒ M1 strains. The SKI8 gene was disrupted and found to be nonessential for cell growth in the absence of M double-stranded RNA (dsRNA). The SKI3 and SKI8 genes were mapped using these clones. We have also obtained other clones suppressing the pathology caused by the high M titer in ski ‒ strains. These clones are not the SKI genes themselves but somehow avoid the growth defect without repressing M copy number.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Ball, S.G., C. Tirtiaux, and R.B. Wickner (1984) Genetic control of L-A and L-(BC) dsRNA copy number in killer systems of Saccharomyces cerevisiae. Genetics 107:199–217.
Cohn, M.S., C.W. Tabor, H. Tabor, and R.B. Wickner (1978) Spermidine or spermine requirement for killer double-stranded RNA plasmid replication in yeast. J. Biol. Chem. 253:5225–5227.
Di Nardo, S., K. Voelkel, and R. Sternglanz (1984) DNA topoisomerase II mutant of Saccharomyces cerevisiae: Topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication. Proc. Natl. Acad. Sci., USA 81:2616–2620.
Gotoh, T., and J.C. Wang (1984) Yeast DNA topoisomerase II is encoded by a single-copy, essential gene. Cell 36:1073–1080.
Hinnebusch, A., and G.R. Fink (1983) Positive regulation in the general amino acid control of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci., USA 80:5374–5381.
Johnston, M., and R.W. Davis (1984) Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae. Mol. Cell. Biol. 4:1440–1448.
Oshima, Y. (1982) Regulatory circuits for gene expression: The metabolism of galactose and phosphate. In The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, J.N. Strathern, E.W. Jones, and J.R. Broach, eds. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 159–180.
Pringle, J.R., and L.H. Hartwell (1981) The Saccharomyces cerevisiae cell cycle. In The Molecular Biology of the Yeast Saccharomyces: Metabolism and Gene Expression, J.N. Strathern, E.W. Jones, and J.R. Broach, eds. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 97–142.
Ridley, S.P., S.S. Sommer, and R.B. Wickner (1984) Superkiller mutations in Saccharomyces cerevisiae suppress exclusion of M2 double-stranded RNA by L-A-HN and confer cold sensitivity in the presence of M and L-A-HN. Mol. Cell. Biol. 4:761–770.
Shriver, K., and B. Byers (1977) Yeast microtubules: Constituent proteins and their synthesis. J. Cell Biol. 75:297a.
Thrash, C., K. Voelkel, S. Di Nardo, and R. Sternglanz (1984) Identification of Saccharomyces cerevisiae mutants deficient in DNA topoisomerase I activity. J. Biol. Chem. 259:1375–1377.
Toh-e, A., and R.B. Wickner (1980) “Superkiller” mutations suppress chromosomal mutations affecting double-stranded RNA killer plasmid replication in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci., USA 77:527–530.
Toh-e, A., P. Guerry, and R.B. Wickner (1978) Chromosomal superkiller mutants of Saccharomyces cerevisiae. J. Bacteriol. 136:1002–1007.
Wickner, R.B. (1978) Twenty-six chromosomal genes needed to maintain the killer double-stranded RNA plasmid of Saccharomyces cerevisiae. Genetics 88:419–425.
Wickner, R.B. (1979) Mapping of chromosomal genes of Saccharomyces cerevisiae using an improved genetic mapping method. Genetics 92:803–821.
Wickner, R.B. (1980) Plasmids controlling exclusion of the K2 killer double-stranded RNA of yeast. Cell 21:217–226.
Wickner, R.B. (1985) Double-stranded RNA replication in yeast: The killer system. Ann. Rev. Biochem. 55:373–395.
Wickner, R.B., and M.J. Leibowitz (1976) Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cerevisiae: The killer character of yeast. J. Mol. Biol. 105:427–443.
Wickner, R.B., and M.J. Leibowitz (1979) mak mutants of yeast: Mapping and characterization. J. Bacteriol. 140:154–160.
Wickner, R.B., S.P. Ridley, H.M. Fried, and S.G. Ball (1982) Ribosomal protein L3 is involved in replication or maintenance of the killer double-stranded RNA genome of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci., USA 79:4706–4708.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Plenum Press, New York
About this chapter
Cite this chapter
Icho, T., Lee, HS., Sommer, S.S., Wickner, R.B. (1986). Molecular Characterization of Chromosomal Genes Affecting Double-Stranded RNA Replication in Saccharomyces Cerevisiae . In: Wickner, R.B., et al. Extrachromosomal Elements in Lower Eukaryotes. Basic Life Sciences, vol 40. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-5251-8_13
Download citation
DOI: https://doi.org/10.1007/978-1-4684-5251-8_13
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-5253-2
Online ISBN: 978-1-4684-5251-8
eBook Packages: Springer Book Archive