On the Composition of Autofluorescent Accumulation Products: Ceroid and Lipofuscin

  • G. J. M. Hooghwinkel
  • A. J. Blaauboer
  • L. Novak
  • L. A. W. Trippelvitz
Part of the NATO ASI Series book series (NSSA, volume 116)

Abstract

Ceroid was isolated post mortem from the brain of a patient, 29 years old, with the juvenile type of neuronal ceroidlipofuscinosis (NCL). Lipofuscins were isolated post mortem from the brains of individuals, 65 years old and 78 years old, free of neurological disorders. The lipofuscins were pooled. The isolated ceroid and the pooled isolated lipofuscins were fractionated with chloroform/methanol 2:1 (v/v) (fraction A), chloroform/methanol/water 10:10:3 (v/v/v) (fraction B), leaving behind a residue (fraction C).

Biochemical analyses of these fractions, of both lipopigments, were performed with special attention to the occurrence of carbohydrates, dolichol and dolichol derivatives.

Keywords

Enzymatic Conversion Lysosomal Storage Disease High Performance Thin Layer Chromatography Mannose Residue Neuronal Ceroidlipofuscinosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • G. J. M. Hooghwinkel
    • 1
  • A. J. Blaauboer
    • 1
  • L. Novak
    • 1
  • L. A. W. Trippelvitz
    • 1
  1. 1.Department of Medical ChemistryVrije UniversiteitAmsterdamThe Netherlands

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